【摘要】 目的：探讨异鼠李素对口腔鳞癌细胞（SCC-9、SAS）增殖、迁移和凋亡的影响及其机制。方法：体外培养SCC-9、SAS细胞，加入不同浓度的异鼠李素，CCK-8法检测细胞活力。将SCC-9、SAS两种细胞分别分为3组：对照组（不予处理）、低剂量组和高剂量组。用平板克隆实验检测细胞集落形成情况，划痕实验和Transwell迁移实验检测细胞水平及垂直迁移情况，流式细胞术检测细胞凋亡，Western blotting法检测PI3K/AKT/mTOR信号通路相关蛋白表达。结果：随着异鼠李素浓度增加，SCC-9、SAS细胞增殖抑制率升高（P<0.05）。与对照组相比，两种细胞的低剂量组和高剂量组克隆形成率和水平迁移率降低，垂直迁移细胞数减少，两种细胞的高剂量组细胞凋亡率升高（均P<0.05）。SCC-9、SAS细胞高剂量组PI3K110、PI3K85、AKT、pAKT、mTOR、p-mTOR、S6、p-S6蛋白表达水平均低于对照组（P<0.05）。结论：异鼠李素可能通过抑制PI3K/AKT/mTOR信号通路抑制口腔鳞癌细胞的增殖、迁移，并促进细胞凋亡。更多还原

【Abstract】 Objective:To investigate the effects and mechanism of isorhamnetin on proliferation,migration and apoptosis of oral squamous cell carcinoma (SCC-9,SAS) cells.Methods:SCC-9 and SAS cells were cultured in vitro,and different concentrations of isorhamnetin were added to detect the cell viability by CCK-8 method.SCC-9 and SAS cells were divided into three groups:control group (no treatment),low-dose group and high-dose group.Cell colony formation was detected by plate cloning assay.The horizontal and vertical migration of cells were detected by scratch and Transwell migration assay.Cell apoptosis was detected by flow cytometry,and protein expressions related to PI3K/AKT/mTOR signaling pathway were detected by Western blotting.Results:With the increase of isorhamnetin concentration,the proliferation inhibition rate of SCC-9 and SAS cells increased (P<0.05).Compared with the control group,the clone formation rate and horizontal migration rate decreased in the low-dose and high-dose groups of the two kinds of cells,the number of vertical migration cells decreased,and the apoptosis rate increased in the high-dose group of the two kinds of cells (all P<0.05).The protein expressions of PI3K110,PI3K85,AKT,p-AKT,mTOR,p-mTOR,S6 and p-S6 in SCC-9 and SAS cell highdose groups were lower than those in the control group (P<0.05).Conclusion:Isorhamnetin may inhibit the pro-liferation as well as the migration and promote the apoptosis of SCC-9 and SAS cells by inhibiting PI3K/AKT/mTOR signaling pathway.更多还原