【摘要】 目的：探索GINS1基因在鼻咽癌（NPC）中的表达情况，并研究沉默GINS1基因是否影响NPC细胞的恶性生物学行为。方法：使用公共数据库GEO，比较GINS1基因在NPC组织和正常鼻咽上皮组织（NNE）中转录水平的表达差异。通过实时荧光定量聚合酶链式反应（RT-qPCR）检测GINS1在转录水平的表达差异。通过蛋白免疫印迹法（Western blotting）检测GINS1在蛋白水平的表达。使用siRNA干扰技术敲低NPC细胞中GINS1基因的表达。分别通过细胞计数试剂盒（CCK-8）法、划痕实验、Transwell侵袭实验检测细胞的增殖、迁移及侵袭能力。结果：基于GEO数据库的生物信息学分析，发现GINS1在NPC中呈高转录水平（SMD=2.23;P<0.05）。GINS1的mRNA表达在NPC细胞系和组织中上调（P<0.05），其表达在患者的年龄、性别、T分期、N分期及临床分期中差异无统计学意义（均P>0.05）。敲低GINS1，显著抑制细胞的增殖、迁移和侵袭能力（均P<0.05）。结论：GINS1是NPC潜在的肿瘤致癌基因，可能作为NPC的有效诊断标志物。更多还原

【Abstract】 Objective: To explore the expression of GINS1 in nasopharyngeal carcinoma(NPC), and to study whether it affects the proliferation, migration, and invasion abilities of NPC cells after knocking down the GINS1. Methods: The GEO database was used for exploring the transcription level of GINS1 between NPC and normal nasopharyngeal epithelia(NNE) samples. Quantitative real-time PCR(RT-qPCR)was used for identifying the differential expression of GINS1 at the transcription level. The protein expression of GINS1 was detected by western blotting. Moreover, GINS1 expression in NPC cells was knocked down by siRNA interference technology. The proliferation, migration, and invasion abilities of NPC cells were assessed through the CCK-8 test,wound healing assay, and transwell assay, respectively. Results: According to the analysis result based on the GEO database, GINS1 showed a high transcription level in NPC when compared with NNE tissue samples(SMD=2.23,P<0.05). The mRNA expression of GINS1 was remarkably upregulated in NPC cell lines and tissue(P<0.05). However, there was no significant difference in the mRNA expression of GINS1 among different clinical groups of age, sex, T stage, N stage,and clinical stage(P>0.05). Knockdown of GINS1 significantly reduced the proliferation, migration, and invasion abilities of NPC cells(P<0.05). Conclusion:GINS1 is a potential tumor oncogene in NPC, and may serve as an effective diagnostic biomarker for NPC.更多还原