【摘要】 目的：研究棒柄花苷A（PPGA）对白介素-1β（IL-1β）诱导活化的大鼠肝星状细胞（HSC-T6）增殖、胶原、凋亡和细胞周期的影响。方法：通过MTT法检测PPGA对HSC-T6细胞活力的影响，计算半数抑制浓度(IC₅₀)，以IC₅₀设置给药浓度。将HSC-T6细胞分为正常对照组、模型组和PPGA高、中、低剂量组，除正常对照组外，其他各组均用IL-1β刺激30 min使HSC-T6细胞活化，建立体外肝纤维化模型。药物干预24 h后，采用MTT法检测HSC-T6细胞增殖活力，免疫荧光法检测Ⅰ型胶原蛋白（Col-Ⅰ）和Ⅲ型胶原蛋白（Col-Ⅲ）表达，流式细胞仪检测细胞凋亡和细胞周期。结果：PPGA作用于HSC-T6细胞24 h、48 h、72 h的IC₅₀分别为473 mg/L、100 mg/L、15 mg/L，因此选择200 mg/L、100 mg/L、50 mg/L分别作为PPGA高、中、低剂量进行后续实验。与正常对照组比较，模型组HSC-T6细胞增殖能力提高，Col-Ⅰ和Col-Ⅲ蛋白表达升高（均P<0.05），而细胞凋亡率和周期分布无明显改变（P>0.05）；与模型组比较，PPGA高、中剂量组HSC-T6细胞增殖活力降低，Col-Ⅲ蛋白表达降低，PPGA各剂量组Col-Ⅰ蛋白表达降低，凋亡率升高，细胞S期延长（均P<0.05）。结论：PPGA可抑制HSC-T6细胞增殖，诱导细胞凋亡，并使HSC-T6细胞周期阻滞在S期，减缓肝纤维化的发展进程。更多还原

【Abstract】 Objective:To investigate the effects of PPGA from Cleidion brevipetiolatum Paxet Hoffm on proliferation,collagen,apoptosis and cell cycle of activated rat hepatic stellate cells （HSC-T6） induced by interleukin-1β（IL-1β）.Methods:The effect of PPGA on the viability of HSC-T6 cells was detected by MTT method.The 50%inhibitory concentration (IC₅₀) was calculated.The drug concentration was set by IC₅₀.HSC-T6 cells were divided into normal control group,model group and high,middle and low dose groups of PPGA.Except for the normal control group,HSC-T6 cells were activated by IL-1βstimulation for 30 min to establish an in vitro model of liver fibrosis.After 24 hours of drug intervention,the proliferation activity of HSC-T6 cells was detected by MTT method,the expression of typeⅠcollagen （Col-Ⅰ） and typeⅢcollagen （Col-Ⅲ） was detected by immunofluorescence,and the apoptosis and cell cycle were detected by flow cytometry.Results:The IC₅₀of HSC-T6 cells treated with PPGA for 24 h,48 h and 72 h was 473 mg/L,100 mg/L and 15 mg/L,respectively,so 200 mg/L,100 mg/L and 50 mg/L were selected as high,middle and low doses of PPGA for follow-up experiments.Compared with the normal control group,the proliferation ability of HSC-T6 cells and the expression of Col-Ⅰand Col-Ⅲproteins in the model group increased （all P>0.05）,while the apoptosis rate and cell cycle distribution had no significant change （P>0.05）.Compared with the model group,the proliferation activity of HSC-T6 cells and Col-Ⅲprotein expression in the high and middle dose groups of PPGA decreased,and the expression of Col-Ⅰprotein decreased,the apoptosis rate increased and the S phase prolonged in all PPGA groups （all P<0.05）.Conclusion:PPGA can inhibit the proliferation of HSC-T6 cells,induce apoptosis,block the HSC-T6 cell cycle in S phase and slow down the development of liver fibrosis.更多还原