【摘要】 目的：探讨舒芬太尼（Sufen）对肾缺血再灌注（RIRI）大鼠肾组织细胞自噬的影响及其作用机制。方法：将60只雄性SD大鼠随机分为对照组、模型组、Sufen组、LY294002组、Sufen+LY294002组，每组12只，除对照组外，其他组均需构建RIRI模型。检测大鼠血清中肌酐（Cr）、血尿素氮（BUN）、丙二醛（MDA）和超氧化物歧化酶（SOD）活性；Western blotting检测大鼠肾脏组织中微管相关蛋白1轻链3(LC3)、p62、磷脂酰肌醇-3-激酶（PI3K）、PI3K、蛋白激酶B(AKT)、p-AKT蛋白表达。结果：与模型组比较，Sufen组大鼠血清中Cr、BUN水平降低，肾脏组织中的肾小管损伤减轻，肾脏组织中MDA含量、自噬小体的数量、LC3-Ⅱ/LC3-Ⅰ降低，SOD活性、p62蛋白、p-PI3K/PI3K、p-AKT/AKT水平升高，而LY294002组上述对应指标与Sufen组呈相反趋势（均P<0.05）；与Sufen组比较，Sufen+LY294002组大鼠血清中Cr、BUN水平升高，肾脏组织中的肾小管损伤严重，肾脏组织中MDA含量、自噬小体的数量、LC3-Ⅱ/LC3-Ⅰ升高，SOD活性、p62蛋白、p-PI3K/PI3K、p-AKT/AKT水平降低（均P<0.05）。结论：舒芬太尼可能通过激活PI3K/AKT通路来抑制过度自噬，进而发挥对RIRI大鼠肾脏的保护作用。更多还原

【Abstract】 Objective: To investigate the effects of sufentanil(Sufen) on the autophagy of renal tissue cells in rats with renal ischemia reperfusion injury(RIRI) and its mechanism. Methods: Sixty male SD rats were randomly divided into control group, model group, Sufen group, LY294002 group and Sufen+LY294002 group, 12 rats in each group. RIRI model was established in all groups except for the control group. Creatinine(Cr), blood urea nitrogen(BUN), malondialdehyde(MDA) and superoxide dismutase(SOD) activities were detected in rat serum.The expressions of microtubule-associated protein 1 light chain 3(LC3), p62, phosphatidylinositol-3-kinase(PI3K), p-PI3K, protein kinase B(AKT) and p-AKT in rat kidney tissue were detected by western blotting. Results: Compared with the model group, the serum levels of Cr and BUN, tubular damage in renal tissues, MDA content, number of autophagic vesicles and LC3-Ⅱ/LC3-Ⅰ in renal tissues were reduced in the Sufen group,whereas the SOD activity, p62 protein level, and p-PI3K/PI3K and p-AKT/AKT ratios were increased, while the corresponding indexes in the LY294002 group showed the opposite trend to those in the Sufen group(P<0.05).Compared with the Sufen group, the rats in the Sufen + LY294002 group had increased serum levels of Cr and BUN, severe tubular damage in renal tissues, increased MDA content, number of autophagic vesicles and LC3-Ⅱ/LC3-Ⅰ,along with decreased SOD activity,p62 pro‐tein level,p-PI3K/PI3K and p-AKT/AKT in renal tis‐sues (P<0.05).Conclusion:Sufentanil may inhibit excessive autophagy by activating the PI3K/AKT pathway,which in turn exerts a protective effect on the kidney of RIRI rats.更多还原