【摘要】 为了提高SiO₂载体材料的酶固定化性能，分别采用接枝法和原位法对自制的分级介孔SiO₂空心微球进行氨基修饰，并考察其木瓜蛋白酶固定化性能。与未修饰的样品相比，接枝法修饰后微球一级最可几孔径（8.68～8.70 nm）减小，二级最可几孔径（29.30～29.89 nm）无明显变化，表明氨基主要修饰在一级孔道中。原位法修饰后样品的一级最可几孔径（4.47～7.34 nm）、二级最可几孔径（14.49～19.78 nm）均有明显减小，表明一、二级孔道中均有氨基修饰。与未修饰和接枝法修饰的样品相比，原位法修饰后样品的最大木瓜蛋白酶固定量为750 mg·g^-1，相对酶活力为121.0%，均高于未修饰和接枝法修饰的样品。原位法氨基修饰在提高SiO₂载体材料的酶固定化性能方面更具优势。更多还原

【Abstract】 In order to improve enzyme immobilization performance of SiO₂ carrier materials,homemade hierarchical mesoporous SiO₂ hollow microspheres were modified with amino groups by grafting and in-situ methods,respectively.Their application in papain immobilization was investigated.Compared with unmodified samples,primary most probable pores （8.68-8.70 nm） of the grafting modified microspheres decrease and secondary most probable pores （29.30-29.89 nm） do not change significantly,which indicates that the amino groups are mainly grafted in primary pores.Both primary （4.47-7.34 nm） and secondary most probable pores（14.49-19.78 nm） decrease significantly after modification via the in-situ method,which indicates that amino groups are modified in both areas.The maximum papain immobilization amount and its corresponding relative enzyme activity of the in-situ modified samples were 750 mg·g^-1 and 121.0%,respectively,which were higher than those modified by grafting and unmodified samples.In-situ amino modification has advantages in improving enzyme immobilization performance of SiO₂ carriers.更多还原