【摘要】 目的：采用超高效液相色谱-四级杆-静电轨道串联高分辨质谱法（UPLC-QE-Orbitrap-MS）对赣南地区产铁皮石斛茎和根水提物的化学成分进行表征。方法：使用Welch Ultimate XB-C₁₈型色谱柱（50 mm×2.1 mm,1.8μm），以甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱，流速为0.3 mL·min^–1；采用电喷雾电离源（ESI），正负离子切换扫描模式进行Full mass/dd-MS₂数据采集，通过mzCloud数据库检索比对和参考已报道文献对赣南地区产铁皮石斛茎和根的化学成分进行表征。结果：结合数据库和文献报道对铁皮石斛茎和根水提物中的29个共有成分、4个根差异成分和1个茎差异成分进行了初步表征，包括有机酚酸类、黄酮类、核苷类、脂肪酸类及酰胺类，并通过铁皮石斛茎和根化学成分进行PCA分析和Venn差异分析，根和茎成分种类较为一致。结论：UPLC-QEOrbitrap-MS法灵敏度高，分析快速，结果准确、可靠，能够全面快速分析和鉴定铁皮石斛不同部位水提物中的化学成分，为其质量控制、进一步药理研究和扩大应用提供了科学依据。更多还原

【Abstract】 Objective :To reveal the chemical constituents characterization of aqueous extracts from stems and roots of Dendrobium officinale Kimura et Migo produced in southern Jiangxi based on UPLC-QE-Orbitrap-MS.Methods :A Welch Ultimate XB-C₁₈ column（50 mm×2. 1 mm,1. 8 μm）was used with methanol as mobile phase A and 0. 1% methanoic acid aqueous solution(0. 3 mL·min^–1)as mobile phase B,the separation and determination of the selected phytohormones were carried out by ultra high-performance liquid chromatography coupled to tandem mass spectrometry（UPLC-MS/MS）,using electrospray ionization source（ESI）in positive and negative ion modes,retrieving comparison and reference through mzCloud database and reported literature.Results :According to retention time,precise molecular weight and characteristic fragmentation ions,29 same chemical components,4 characteristic chemical compositions of root and 1characteristic chemical composition of stem were tentatively characterized and identified from the stem and root of Dendrobium officinale Kimura et Migo by comparing with mzCloud mass spectral library and published references,mainly including flavonoids,organic and phenolic acids,nucleosides,fatty acids and amides. The stems and roots were similar in chemical composition.Conclusion :This method can be used for rapid and comprehensive qualitative analysis of the chemical constituents of different parts from Dendrobium officinale Kimura et Migo produced,which provides references for the quality control and comprehensive utilization of Dendrobium officinale.更多还原