【摘要】 目的：探讨Ca²⁺对转化生长因子β₁诱导的系膜细胞自噬和细胞外基质蛋白表达的影响。方法：通过Western blot方法研究Ca²⁺对转化生长因子β₁诱导的系膜细胞胞外基质蛋白FN及自噬相关蛋白mTOR、P70S6K、Beclin-1和P62的表达变化。结果：TGF-β₁和Ca²⁺共处理系膜细胞24 h后，通过Western blot检测发现TGF-β₁刺激系膜细胞可抑制mTOR/p70S6K的磷酸化和P62的表达，上调Beclin-1和胞外基质蛋白FN的表达，Ca²⁺和TGF-β₁共同处理可以上调mTOR/p70S6K的磷酸化和P62的表达，同时下调Beclin-1的表达，并且抑制TGF-β₁诱导的FN表达。结论：在系膜细胞中钙离子能抑制TGF-β₁诱导的自噬和胞外基质蛋白表达。更多还原

【Abstract】 Objective:To investigate the effect of Ca²⁺on transforming growth factorβ₁-induced autophagy and extracellular matrix expression in mesangial cells.Methods:The expression of extracellular matrix protein FN,and changes in the expression of autophagy-related proteins mTOR,P70S6K,Beclin-1 and P62 were investigated by Western Blot.Results:The results showed that mesangial cells co-treated with TGF-β₁and Ca²⁺for 24 hours,TGF-β₁stimulation of mesangial cells could inhibit the phosphorylation of mTOR/p70S6K and the expression of P62,up-regulate Beclin-1and extracellular matrix protein FN.Ca²⁺and TGF-β₁co-treatment could up-regulate the phosphorylation of mTOR/p70S6K and the expression of P62.At the same time,it decreased the expression of Beclin-1 and inhibited TGF-β₁induced FN expression.Conclusion:In mesangial cells calcium can inhibit autophagy and extracellular matrix protein expression induced by TGF-β₁.更多还原