【摘要】 目的：探讨DigiGait成像系统评估帕金森病（PD）模型大鼠步态变化的可行性。方法：选择清洁级雄性SD大鼠24只，按照随机数字表法分为正常组、假手术组及模型组，每组8只。模型组采用单侧2点法脑注射6-羟基多巴胺（6-OHDA）制备PD大鼠模型，假手术组运用相同方法脑注射0.2%抗坏血酸溶液，正常组不进行任何手术处理。采用DigiGait成像系统分析3组大鼠跑带运动时间子成分（摆动时长、制动时长、触地时长、站立系数）、空间子成分（步幅频率、步幅长度、触地宽度、步态对称性、脚爪重叠距离、脚爪放置定位）及姿势子成分（脚爪角度、步偏角、脚爪面积）等3个维度的步态变化。结果：(1)时间子成分：与正常组比较，模型组左前肢、左后肢及右前肢在运动过程中的摆动时长、制动时长及触地时长明显延长（P<0.05），右后肢制动时长及摆动时长明显延长（P<0.05）。与假手术组比较，模型组左前肢、左后肢及右前肢在运动过程中的摆动时长、制动时长及触地时长较长（P<0.05），右后肢制动时长较长（P<0.05）。(2)空间子成分：与正常组和假手术组比较，模型组前后肢触地宽度明显增加（P<0.05），四肢的步幅频率、左侧肢步幅长度及大鼠的脚爪放置位置与步态对称性显著降低（P<0.05），但右侧肢步幅长度和左右侧肢的脚爪重叠距离无明显变化（P>0.05）。(3)姿势子成分：与正常组和假手术组比较，模型组在运动过程中左前肢、左后肢及右后肢与跑带接触的脚爪面积较小（P<0.05），右前肢与跑带接触的脚爪面积、脚爪角度及步偏角差异均无统计学意义（P>0.05）。结论：DigiGait成像系统可以客观评价PD模型大鼠步行功能、平衡功能及协调功能，在PD相关基础研究中具有广泛应用的前景。更多还原

【Abstract】 Objective: To detect the feasibility of Digi Gait ^TM Imaging System in evaluating gait changes in rats model with Parkinson’s disease （PD）. Methods: A total of 24 clean male SD rats were randomly divided into normal group, sham operation group and model group, with eight rats in each group. In the model group, 6-Hydroxydopamine （6-OHDA） was injected into the brain with unilateral 2-point method to establish PD rats model. The sham operation group was injected with 0.2% ascorbic acid so‐lution with the same method, and the normal group did not receive any surgical treatment. After successful preparation of the model,Digi Gait^TMImaging System was used to analyze temporal subcomponent （swing duration, braking duration, stance duration, stance factor）, spatial subcomponent （stride frequency, stride length, stance width, gait symmetry, paw overlap distance, paw placement po‐sitioning） and postural subcomponent （paw angle, step angle, paw area）. Results: 1) Temporal subcomponent: compared with the normal group, the swing duration, braking duration and stance duration of left front paw, left hind paw and right front paw in the model group were significantly prolonged （P<0.05）, and the braking duration and swing duration of right hind paw in the model group were significantly prolonged （P<0.05）. Compared with the sham operation group, the swing duration, braking duration and stance duration of left front paw, left hind paw and right front paw in the model group were significantly prolonged （P<0.05）, and the braking duration of right hind paw in the model group was significantly prolonged （P<0.05）. 2) Spatial subcomponent: compared with the normal group and the sham operation group, the front and hind limb stance width of the model group significantly increased（P<0.05）, step frequency of four paws, stride length of left paws and paw placement positioning and gait symmetry in the model group significantly decreased （P<0.05）. But stride length of right paws and overlap distance of left and right paws in the model group had no significant changes （P>0.05）. 3) Postural subcomponent: compared with the normal group and the sham operation group, the contact area of left front paw, left hind paw and right hind paw with running belt in the model group significantly de‐creased （P<0.05）, there was no significant difference in paw area, paw angle and gait angle between the right front paw and the run‐ning belt （P>0.05）. Conclusion: Digi Gait^TMImaging System can objectively evaluate walking, balance and coordination functions of PD rats, which has the potential to be widely applied in PD related basic research.更多还原