【摘要】 目的 建立了一种毛发中26种毒品及其代谢物的UPLC-MS/MS快速定性定量检测方法。方法 毛发经酸化甲醇（pH=3）水浴超声提取后，ACQUITY UPLC?BEH C18色谱柱分离，乙腈（0.1%甲酸）-水（0.01%甲酸+0.05%氨水，体积比）梯度洗脱，电喷雾离子源正离子（ESI+）及选择反应监测模式（SRM）检测。结果 26种毒品及代谢物在0.1～40.0 ng/mg范围内线性关系良好，相关系数R2均> 99.5%；方法检出限（S/N=3）在0.03～0.06ng/mg间、定量限（S/N=10）在0.1～0.2 ng/mg间；回收率（0.1 ng/mg, 1.0 ng/mg, 10.0 ng/mg）在68.3%～105.6%间；基质效应在84.0%～114.6%间；相对标准偏差（RSD%,n=6）在1.2%～8.3%之间；日间精密度为0.5%～8.9%。结论 本方法毛发使用量低、耗时短，与平行比较的其他提取溶剂具有更高的提取效果，现已应用于毛发检材量不足的实际案件的检验中。更多还原

【Abstract】 Objective To establish a rapid qualitative and quantitative detection method based on UPLC-MS/MS for 26 drugs and their metabolites in human hair. Methods The hair was extracted with acidified methanol(pH = 3) in a water bath, separated on an ACQUITY UPLC? BEH C18 column, gradient eluted with acetonitrile(0.1 % formic acid,volume ratio)-water(0.01 % formic acid+0.05 % ammonia, volume ratio), and detected with electrospray positive ionization and SRM mode. Results All the linear ranges for the 26 drugs and their metabolites were 0.1 ～ 40.0 ng/mg, and all the correlation coefficients were more than 0.995. The limits of detection(S/N = 3) were between 0.03 and 0.06 ng/mg and the limits of quantification(S/N = 10) were between 0.1 and 0.2 ng/mg. The recoveries(0.1 ng/mg, 1.0 ng/mg, 10.0 ng/mg)were in the range of 68.3 % to 105.6 %, and the matrix effects were in the range of 84.0 % to 114.6 %. The relative standard deviations(RSD, n=6) ranged from 1.2 % to 8.3 %, and the inter-day precision was ranged from 0.5 % to 8.9 %. Conclusion This method is low hair-consumption and short time-consuming, and has higher extraction effects compared with other parallel extraction methods. It has been widely used in the inspection of actual cases with insufficient hair samples.更多还原