【摘要】 从某化工厂污水处理车间好氧曝气池的活性污泥中，筛选了一株对非离子表面活性剂Triton X-100具有降解能力的菌株ISB5。对菌株的16S rDNA进行测序和比对分析，将菌株ISB5鉴定为假单胞菌属（Pseudomonas sp.）。进一步对菌株ISB5降解Triton X-100的培养条件进行考察，得到了菌株的适宜降解条件：培养温度为30℃，Triton X-100的初始质量浓度为4 g/L，培养时间为28 h，振荡速率为150 r/min，装液量为100.00mL，接种量为1.00%（菌株ISB5与培养基的体积比）。在该条件下，TritonX-100的降解率可达93.0%以上。更多还原

【Abstract】 A strain ISB5 with the ability to degrade the non-ionic surfactant Triton X-100 was screened from the activated sludge in the aerobic aeration tank of a sewage treatment plant in a chemical plant. Through sequencing and comparison analysis of the16S rDNA of the strain, the strain ISB5 was identified as Pseudomonas sp. The culture conditions of strain ISB5 to degrade Triton X-100 were further investigated, and the suitable degradation conditions of the strain were obtained: Culture temperature is 30 ℃,initial concentration of Triton X-100 is 4 g/L, culture time is 28 h, shaking rate is 150 r/min, filling volume is 100.00 mL,inoculation the amount is 1.00%(volume ratio of strain ISB5 to medium). The degradation rate of TritonX-100 by the strain under this condition can reach more than 93.0%.更多还原