【摘要】 【目的】了解福建地区鸡传染性支气管炎病毒（Infectious bronchitis virus,IBV）的流行及其S1基因变异情况。【方法】对2021年在福建地区鸡群中分离的2株病毒，通过鸡胚致病性试验、电子显微镜观察及RT-PCR方法进行鉴定。对2株分离株的S1基因进行克隆、测序并利用生物学软件进行分析。【结果】分离到的2株病毒均为IBV，分别命名为FJ-NP01和FJ-FZ01;FJ-NP01株和FJ-FZ01株的S1基因全长分别为1 629 nt和1 620 nt，编码543aa和540 aa。FJ-FZ01株的S1基因裂解位点为HRRRR，与基因型I分支所有参考毒株裂解位点一致；分离株FJNP01的S1基因裂解位点为HRRKR，与已报道的基因型ⅣIBV分离毒株的裂解位点不同，与基因型Ⅵ参考毒株TC07-2的裂解位点一致。FJ-NP01株和FJ-FZ01株之间的核苷酸和氨基酸同源性较低，分别为83.2%和79.6%；与中国使用的Mass型常规疫苗H120和H52核苷酸和氨基酸序列同源性仅为75.7%～76.3%和77.1%～83.5%。FJ-NP01株是由基因型Ⅳ毒株CK CH GD LZ12-4与基因型I毒株L-1 148在S1基因处发生重组而产生的新毒株，其核苷酸序列1 438～1 506 nt与推测的亲本毒株CK CH GD LZ12-4同源性达97%，其他S1基因核苷酸序列与推测的亲本毒株L-1148同源性达95.9%。【结论】以上结果表明福建地区IBV流行较为复杂，现有Mass型疫苗在福建省可能起不到良好的免疫保护作用。更多还原

【Abstract】 【Objective】 Epidemiology and genetic variations of the infectious bronchitis virus(IBV) in Fujian province were studied. 【Method】 Two strains of virus isolated from the diseased chickens in Fujian in 2021 were identified by chicken embryo pathogenicity test, electron microscope observation, and RT-PCR. S1 genes of the isolates were cloned, sequenced, and analyzed using biological software. 【Result】 The two IBV strains were code named FJ-NP01 and FJ-FZ01. The full length of S1 of FJ-NP01 was 1 629 nt encoding 543 amino acids, and that of FJ-FZ01, 1 620 nt encoding 540 amino acids. The S1 gene cleavage site of FJ-FZ01 was HRRRR, same as all reference strains of genotype I branch; while that of FJ-NP01 HRRKR differed from the reported site of IBV isolated from genotype Ⅳ but same as that of TC07-2 reference strain of genotype Ⅵ.The homology of nucleotide and amino acid between the two isolates was 83.2% and 79.6%, respectively, but merely 75.7%–76.3%and 77.1%–83.5% with the Mass-type conventional vaccines H120 and H52, respectively. Further analysis showed that FJ-NP01was from a recombination event between CK CH GD LZ12-4 and L-1148, the homology of nucleotide acid between 1438–1506 nt of FJ-NP01 with CK CH GD LZ12-4 was 97%, and 95.9% between the other nucleotide acid of S1 gene with L-1148.【Conclusion】 It appeared that the IBV epidemic experienced in the province was complex in nature and that the existing Mass vaccines would not provide sufficient immune protection to deter the spread.更多还原