【摘要】 设立福建农林大学校园内17个水域位点，进行逐月采样，分离培养大肠杆菌和肠球菌并测定其对几种常用抗生素的耐药性.结果表明，在水环境中有74.52%的分离菌存在不同程度的耐药情况.其中肠球菌对利福平耐药率最高(65.82%),对万古霉素和环丙沙星较敏感，耐药率分别为5.06%和7.59%;大肠杆菌对四环素耐药率最高(43.59%),对环丙沙星和呋喃妥因较为敏感，耐药率均为7.69%.总体而言，9—10月环境指示菌的耐药程度高于11—12月.在17个采样点中，受污水影响较强的湿地公园和观音湖地区分离的细菌耐药性更强，并且较多为多重耐药菌株.更多还原

【Abstract】 To study the spatiotemporal variation of antibiotic resistance of water body on campus, water and sediments were sampled from 17 water sampling points across Fujian Agriculture and Forestry University campus from September to December in 2020. Two environmental indicator bacteria, Escherichia coli and Enterococcus, were isolated and cultured, and their resistances to several common antibiotics were evaluated based on minimal inhibitory concentration. The results showed that 74.52% of the isolates from school water environment had drug resistances to some extent. Enterococci showed the highest resistance to rifampicin(65.82%) while they were relatively sensitive to vancomycin and ciprofloxacin, resistance rates 5.06% and 7.59%, respectively. E.coli showed the highest resistance to tetracycline(43.59%) while being sensitive to ciprofloxacin and nitrofurantoin, with resistance rate being 7.69% for both. In general, the antibiotic resistances of E.coli and Enterococcus were higher in September and October than those in November and December; among the 17 sampling points, water samples from Wetland Park and Guanyin Lake showed higher resistances due to the adverse impacts from sewage, and many of them were multi-drug resistant strains.更多还原