【摘要】 蚯蚓生物滤池(VF)是一种生态型减量和稳定剩余污泥的技术，但在连续污泥流胁迫下，蚯蚓的摄食对细菌群落及病原菌的影响尚不明确。文章通过荧光定量PCR和16S r RNA基因测序技术，探究蚯蚓生物滤池内蚯蚓肠道（VEI）及牛粪饲养的蚯蚓肠道（CEI）细菌群落的组成和变化特征，研究连续污泥流胁迫条件对蚯蚓肠道微生物群的影响，并进一步分析蚯蚓肠道对病原菌的抑制贡献。结果表明：（1）VEI较CEI具有更高的菌群丰富度且优势菌门、菌属种类及丰度不同，蚯蚓肠道运输过程中所观察到的选择效应的差异更明显；（2）蚯蚓肠道对不同食源中典型病原菌抑制作用不同，VEI和CEI对沙门氏菌的去除率分别为52.49%、33.79%，前者对克雷伯氏菌、肠球菌、大肠杆菌等削减作用更明显。（3）微生物网络分析表明VEI竞争抑制较CEI更剧烈，相关性分析显示蚯蚓肠道优势菌门、属的竞争抑制对其他多种病原菌属的末端削减起重要作用。蚯蚓肠道显著改变了摄入污泥及牛粪的细菌群落结构，降低了部分病原菌的相对丰度，研究结果可为蚯蚓生物滤池及蚯蚓堆肥效能分析及推广提供参考。更多还原

【Abstract】 The vermifilter(VF), a kind of ecological technology, can be used for reducing and stabilizing excess sludge of wastewater treatment plants; however, the effects of earthworms’ feeding on bacterial communities and pathogens are yet unclear under the continuous stress of excess sludge. In the present paper, a VF pilot experiment was conducted, with the fluorescence qPCR and 16S rRNA gene sequencing technology to investigate the composition and variation of bacterial community in the VF’s earthworm intestine(VEI) and in the control earthworm intestine(CEI) which was cultivated with cow dung, and to study the effect of continuous sludge flow on the bacterial community of earthworm intestine, and furthermore to analyze the contribution of earthworm intestine to the inhibition of pathogenic bacteria. The experiment presented: there were more diverse and richer bacterial communities in VEI than in the CEI, and it appeard that the difference of the selection effects observed during the intestinal transport of vermifiltration earthworms was even more evident; The inhibitory effects of earthworm intestine on typical food-borne pathogens such as Klebsiella, Enterococcus, Escherichia and so on, were different, the removal rates of Salmonella by VEI and CEI being 52.49% and 33.79%, respectively; and microbial network analysis showed that the competitive inhibition of VEI was more violent than that of CEI, and correlation analysis revealed that the competitive inhibition of dominant bacteria in the VEI played a vital role in the terminal reduction of other pathogens. Obviously, the intestine of earthworms remarkably changed the bacterial community structures of ingested sludge and cow dung, and reduced the relative abundance of some pathogens. Hence, the research results could provide references for the analysis and promotion of vermifilter and vermi-composting.更多还原