【摘要】 为消除医药化工废水高盐度及硝基化合物、杂环芳烃对微生物脱氮的抑制影响，该文以含有杂环化合物医药废水的处理厂活性污泥为对象，从中筛选对有毒有机物耐受的菌株X4。根据其系统发育和表型遗传鉴定为Acinetobacter haemolyticus。探究该菌株异养硝化-好养反硝化特性及在不同碳源、C/N、温度、转速、pH条件下的脱氮效能。结果表明，菌株X4在硝化、反硝化培养基中24 h内氨氮、硝酸盐氮去除率分别为99.14%、90.95%，在实际高盐难降解医药废水处理中氨氮去除率也有52.62%,36 h内对废水COD降解速率达到93.33 mg/(L·h)。当菌株X4在以碳源为丁二酸钠、C/N为12、温度为30℃、pH为9、转速为170 r/min条件时脱氮效果最佳。菌株X4对温度及p H耐受范围广，对C/N要求低，在高盐医药化工废水处理方面具备应用价值。更多还原

【Abstract】 This study is dedicated to eliminating the inhibitory effects on the treatment of pharmaceutical wastewater, which is containing high salinity, nitro-compounds and heterocyclic aromatic hydrocarbons. As the inhibitory effect mainly exerted on the microbial denitrification of wastewater process, the activated sludge, used as a study target, was taken from a wastewater treatment plant handling pharmaceutical wastewater containing heterocyclic compounds, and the strain named X4 being tolerant to toxic organics was screened from it. Strain X4 was identified as Acinetobacter haemolyticus by way of the method of phylogeny and phenotypic genetics. For exploring the heterotrophic nitrification-aerobic denitrification characteristics of the strain and its denitrification efficiency under the condition of different carbon sources, C/N, temperatures, rotational speeds, and pH, the incubation experiment was conducted which revealed the removal rates of ammonia nitrogen and nitrate nitrogen in the nitrification and denitrification culture media of strain X4 were respectively 99.14% and 90.95% in 24 h. When strain X4 was adopted in the treatment(on a laboratory experiment scale) of high-salt refractory wastewater taken from a pharmaceutical plant, the result of the experiment in 36 h showed the removal rate of ammonia nitrogen was 52.62%, the COD degradation rate reached 93.33 mg/(L·h). Strain X4 had the best denitrification effect when the carbon source was sodium succinate, C/N was 12, temperature 30 ℃, pH 9, and rotation speed 170 r/min; besides, it showed wide tolerance to temperature and pH, low requirement of C/N. So, it is expected that strain X4 could be applied in the treatment of high salt pharmaceutical and chemical wastewater.更多还原