【摘要】 目的:探讨五味子甲素对脂多糖诱导小鼠RAW264.7细胞的抗炎作用。方法:检测五味子甲素对脂多糖诱导小鼠RAW264.7细胞的增殖、凋亡、吞噬及细胞内白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)和环氧合酶-2(COX-2)水平的影响。脂多糖诱导后加入不同浓度五味子甲素，CCK8法测定RAW264.7细胞增殖能力，中性红试剂检测诱导后RAW264.7细胞的吞噬能力。酶联免疫吸附试验(ELISA)法测定细胞中IL-1β、TNF-α、PGE2和COX-2水平。Annexin V-FITC/PI双染检测五味子甲素对诱导后RAW264.7细胞凋亡的影响。结果:五味子甲素可明显抑制脂多糖诱导小鼠RAW264.7细胞的增殖、吞噬能力及诱导刺激的细胞凋亡；ELISA法测定结果显示，五味子甲素可抑制RAW264.7细胞IL-1β、TNF-α、PGE2和COX-2的分泌。结论:五味子甲素对脂多糖诱导的小鼠RAW264.7细胞炎症具有明显的抑制作用，其机制可能与抑制炎症细胞的增殖、吞噬，减少炎症因子释放，减轻脂多糖诱导的细胞凋亡有关。更多还原

【Abstract】 Objective: To probe into the anti-inflammatory effects of schisandrin A on lipopolysaccharide-induced RAW264.7 cells in mice. Methods: Effects of schisandrin A on proliferation, apoptosis and phagocytosis of lipopolysaccharide-induced RAW264.7 cells in mice and levels of interleukin(IL)-1β, tumor necrosis factor-α(TNF)-α, prostaglandin E2(PGE2) and cyclooxygenase(COX)-2 levels were detected. The proliferation of RAW264.7 cells was measured by CCK8 method and the phagocytosis of RAW264.7 cells was detected by neutral red reagent. IL-1β, TNF-α, PGE2 and COX-2 were determined by enzyme-linked immunosorbent assay(ELISA). Annexin V-FITC/PI double staining was used to detect the effects of schisandrin A on apoptosis of RAW264.7 cells after induction. Results: Schisandrin A could significantly inhibit the proliferation, phagocytosis and apoptosis of lipopolysaccharide-induced RAW264.7 cells in mice. ELISA results showed that schisandrin A could inhibit the secretion of IL-lβ, TNF-α, PGE2 and COX-2 in RAW264.7 cells. Conclusion: Schisandrin A has significant anti-inflammatory effects on lipopolysaccharide-induced RAW264.7 cells in mice, and its mechanism may be related to inhibiting the proliferation and phagocytosis of inflammatory cells, reducing the release of inflammatory factors, and reliefing the lipopolysaccharide-induced apoptosis.更多还原