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水稻F1小孢子培养再生DH系的耐盐性鉴定及稻米品质分析

Assessment of Salt Tolerance and Grain Quality of the DH Lines Regenerated from F1 Microspore Culture in Rice

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【作者】 代金英郭桂梅程新杰刘成洪岳红亮张梦龙宛柏杰孙一标孙明法

【Author】 DAI Jin-ying;GUO Gui-mei;CHENG Xin-jie;LIU Cheng-hong;YUE Hong-liang;ZHANG Meng-long;WAN Bai-jie;SUN Yi-biao;SUN Ming-fa;Jiangsu Coastal Area Institute of Agricultural Sciences;Institute of Biotechnology, Shanghai Academy of Agricultural Sciences, Shanghai Municipal Key Laboratory of Agricultural Genetics and Breeding;

【通讯作者】 孙明法;

【机构】 江苏沿海地区农业科学研究所上海市农业科学院生物技术研究所/上海市农业遗传育种重点实验室

【摘要】 以水稻种质M8G与韭菜青杂交的F1代经小孢子培养技术得到的34份DH株系为供试材料,进行耐盐性鉴定及稻米品质分析。结果表明:对DH株系及其亲本进行分蘖期—成熟期耐盐性评价,耐盐等级达1级的材料1份,达3级的材料10份,达5级的材料12份,达7级的材料12份,达9级的材料1份。对DH株系及其亲本的稻米品质性状进行分析,DH株系及其亲本的水份、蛋白质质量分数、直链淀粉质量分数、透明度、垩白粒率、垩白度的变异系数分别为2.00%、13.00%、6.00%、43.03%、48.96%、88.85%。水稻DH株系及其亲本的稻米品质性状之间具有一定的相关性。通过聚类分析将36份材料的品质性状分成4大类。第Ⅳ类材料直链淀粉和蛋白质质量分数最高。综合DH株系及其亲本分蘖期—成熟期耐盐性评价和稻米品质分析发现,分蘖期—成熟期耐盐性较好的材料大部分在第Ⅳ类。

【Abstract】 Rice germplasm M8G was crossed with the rice variety Jiucaiqing, and the resultant F1plants were subjected to microspore culture, generating 34 doubled haploid(DH) lines, which were assessed for salt tolerance and grain quality of rice. The DH lines and their parents were evaluated for salt tolerance from tillering stage to maturity stage. The results showed that one line had the very strong salt tolerance of level 1, ten lines had the strong salt tolerance of level 3, 12 lines showed the moderate salt tolerance of level 5,another 12 lines had the weak salt tolerance of level 7, and another line showed the very weak salt tolerance of level 9. Moreover, the DH lines and their parents varied in grain quality traits, whose coefficients of variation of water content, protein content, amylose content, transparency, chalky grain rate, and chalky degree stood at 2.00%, 13.00%, 6.00%, 43.03%, 48.96%, and 88.85%,respectively. On the other hand,there was a certain degree of correlations between the grain quality traits of the DH lines and their parents. The grain quality traits of the 36 lines were divided into four classes by clustering analysis, of which Class Ⅳ had the highest amylose content and protein content. Based on the evaluation of the DH lines and their parents for salt tolerance from tillering stage to maturity stage and grain quality traits, it was found that the lines with higher salt tolerance from tillering stage to maturity stage mainly belong to Class Ⅳ of rice quality traits.

【基金】 上海市农业遗传育种重点实验室2022年度开放课题(KFKT2022-1);江苏省种业振兴揭榜挂帅项目(JBGS [2021]040-2);盐城市绿色高品质耐盐粳稻工程技术中心(YC2021299)
  • 【文献出处】 大麦与谷类科学 ,Barley and Cereal Sciences , 编辑部邮箱 ,2022年06期
  • 【分类号】S511
  • 【下载频次】10
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