【摘要】 采用水热法制备层状双氢氧化物（layered double hydroxide, LDH）纳米片，将改性聚乙二醇（H₂N—PEG—NH₂）修饰在LDH表面，并进一步修饰靶向肽B3int,最后负载抗癌药物阿霉素（DOX）和光热剂吲哚菁绿（ICG）,构建了一种新的DOX-ICG@LDH-PEG-B3int载药系统。通过扫描电子显微镜、紫外-可见分光光度计、热重分析仪等仪器对材料的形貌和理化性质进行表征，结果表明，DOX-ICG@LDH-PEG-B3int具有良好的形态分布，平均直径为77.68 nm。体外释药、体外细胞摄取、体外细胞毒性试验结果表明，DOX-ICG@LDH-PEG-B3int具有pH和近红外（NIR）双重刺激响应药物释放的性能，可以主动靶向肿瘤细胞，并可以显著提高DOX对细胞的杀伤作用以及ICG的光热效果。更多还原

【Abstract】 Hydrothermal method was used to prepare layered double hydroxide（LDH） nanosheets. Modified polyethylene glycol（H₂N—PEG—NH₂） was modified on the surface of LDH and further modified to the target peptide B3 int. A new DOX-ICG@LDH-PEG-B3 int drug delivery system was constructed by loading the anticancer drug doxorubicin（DOX） and photothermal agent indocyanine green（ICG）. The morphology and physicochemical properties of the materials were characterized by scanning electron microscope, UV-vis spectrophotometer, thermogravimetric analyzer and other instruments. The results show that DOX-ICG@LDH-PEG-B3 int has a good morphological distribution, with an average diameter of 77.68 nm. In vitro drug release, in vitro cell uptake and in vitro cytotoxicity tests indicate that DOX-ICG@LDH-PEG-B-3 int has the pH and near-infrared（NIR） in response to drug release, which can actively target tumor cells, significantly improve the killing effect of DOX on cells and the photothermal effect of ICG.更多还原