【摘要】 基于高通量测序技术，分析河南省栾川县引种栽培大花黄牡丹（Paeonia ludlowii）植株地下0～10 cm与10～20 cm土层根际细菌、根际真菌、丛枝菌根真菌（Arbuscular mycorrhiza fungus,AMF）多样性和群落结构，探讨土壤理化性质与根际微生物群落相关性。结果表明，引种栽植地大花黄牡丹根际微生物资源丰富，根际细菌与真菌分别得到9 989与2 212个可操作分类单元（Operational taxonomic units,OTU）；根际细菌分属于26门67纲512属，变形菌门（Proteobacteria）相对丰度最高；根际真菌分属于15门58纲712属，子囊菌门（Ascomycota）与担子菌门（Basidiomycota）为优势类群。根系与根际土中共提取到116个AMF OTUs，分属于球囊菌门（Glomeromycota）球囊菌纲（Glomeromycetes）4目7科10属，近明球囊霉属（Claroideoglomus）与隔球囊霉属（Septoglomus）为优势类群；基于Bray-Curtis距离非度量多维尺度分析（Non-metric multidimensional scaling,NMDS），根系和根际土AMF群落结构差异较大。根据α多样性指数和NMDS分析结果，3类根际微生物群落在不同深度差异均不显著。Mantel分析结果表明，土壤有机质、铵态氮、速效钾含量和pH对根际微生物群落有显著影响。更多还原

【Abstract】 Based on high-throughout sequencing technology, the diversity and community structure of rhizosphere bacteria, rhizosphere fungi and arbuscular mycorrhizal fungi(AMF) in 0-10 cm and 10-20 cm soil layers of Paeonia ludlowii introduced and cultivated in Luanchuan County, Henan Province were analyzed. The correlation among soil physical and chemical properties and rhizosphere microbial community was discussed. The results showed that the rhizosphere microbial resources of P. ludlowii were abundant, a total of 9 989 operational taxonomic units(OTUs) were obtained from bacteria and 2 212 OTUs were obtained from fungi, respectively. Rhizosphere bacteria belonged to 26phyla, 67 classes and 512 genera, and the Proteobacteria had the highest relative abundance.Rhizosphere fungi belonged to 15 phyla, 58 classes and 712 genera, Ascomycota and Basidiomycota were the dominant groups. A total of 116 AMF OTUs were extracted from roots and rhizosphere soil,which belonged to four orders, seven families and 10 genera of Glomeromycetes. Claroidoglomus and Septoglomus were the dominant groups. Non-metric multidimensional scaling(NMDS) analysis based on Bray Curtis distance showed there were great differences in AMF community structure between root and rhizosphere soil. The α-diversity index and NMDS analysis showed there was no significant difference among the three kinds of rhizosphere microbial community at different depths. Mantel analysis showed that soil organic matter, ammonium-nitrogen, available K contents and pH had significant effects on rhizosphere microbial community.更多还原