【摘要】 豫东肉山羊是河南省以波尔山羊为父本，槐山羊或马槐杂交山羊为母本培育的新品种，在组建豫东肉山羊核心群的过程中，由于历史原因，存在亲缘记录不完整问题。为此,采用12对国际通用微卫星引物对豫东肉山羊核心群内310只（300只母羊，10只公羊）健康个体进行微卫星（SSR）分析，并运用生物信息学方法进行遗传多样性参数统计和家系构建。结果显示，采用12个微卫星位点，在豫东肉山羊核心群中共发现144个等位基因，平均等位基因数11.8个。其中等位基因数量最多的微卫星位点是CSSM66，等位基因数26个，等位基因数量最少的微卫星位点是BMS0812，等位基因数5个。遗传多样性分析结果表明, 12个微卫星位点的平均多态含量（PIC）0.646，其中位点BM1329、BMS0812、BM6404属于中度多态位点，剩余9个位点均属于高度多态位点，豫东肉山羊核心群体平均期望杂合度（He）0.683，平均观测杂合度（Ho）0.625。根据不同公畜的亲缘关系分为7个父畜家系，可囊括185只母畜，另外115只母畜划分至无家系类别。综上, SSR家系的构建，对于厘清群体亲缘关系和科学扩繁具有一定的应用价值和实践意义。更多还原

【Abstract】 Yudong meat goat is a new breed bred in Henan province with Boer goat as the male parent and Huai goat,Huai goat hybrid and Horse head goat as the female parent.In the process of establishing the core group of Henan meat goat,due to historical reasons,there are incomplete kinship records.In this study,12 pairs of international universal microsatellite primers were used to conduct microsatellite(SSR)analysis on 310 healthy individuals(300 ewes,10 rams)in the core group of Yudong meat goats,and used bioinformatics methods to conduct genetic diversity parameter statistics and pedigree construction.The results showed that a total of 144 alleles were found in 12 microsatellite loci in the core group of Yudong meat goats,and the average number of alleles was 11.8.The microsatellite locus with the largest number of alleles was CSSM66,with 26 alleles,and the microsatellite locus with the smallest number of alleles was BMS0812,with 5 alleles.The results of genetic diversity analysis showed that the average polymorphic content (PIC)of 12 microsatellite loci was 0.646,of which loci BM1329,BMS0812 and BM6404 were moderately polymorphic loci,and the remaining 9 loci were highly polymorphic.The average expected heterozygosity(He)of the Yudong meat goat core population was 0.683 and the average observed heterozygosity(Ho)was0.625.According to the kinship of different male animals,it was divided into 7 paternal animal families,which could include 185 female animals,and the other 115 female animals were classified into the category of no family.In Summary,the construction of SSR family had certain application value and practical significance for clarifying group kinship and scientific propagation.更多还原