【摘要】 为探究丹参多糖（SMPs）在奶牛乳腺炎中的应用价值，本研究以分娩后7 d小鼠为对象，SMPs溶液灌胃1周后使用脂多糖（LPS）构建小鼠乳腺炎的模型，24 h后处死小鼠收集乳腺组织检测。H&E结果表明，脂多糖会诱导乳腺组织发生严重的炎症反应；ELISA结果显示，SMPs用药组中TNF-α, IL-6和IL-1β在乳腺组织表达水平显著下降。Western Blot结果显示，SMPs用药组中TLR4,MyD88和NF-κB蛋白表达水平显著降低。RT-qPCR结果显示，SMPs用药组中TNF-α, IL-6和IL-1β mRNA转录水平显著降低。结果表明，SMPs对LPS诱导的乳腺炎症具有保护作用，其抗炎作用与抑制TLR4信号通路中关键蛋白表达水平有关。更多还原

【Abstract】 In order to explore the application value of Salvia miltiorrhiza polysaccharides(SMPs) in dairy mastitis, mice after 7 days delivery were used as subjects in this study. Mouse mastitis model was established using lipopolysaccharide(LPS)after intragastric administration of SMPs solution for 1 week, and mice mammary tissues were collected after sacrifice at 24h of LPS treatment. H&E results showed that LPS could induce severe inflammatory response in breast tissue. ELISA results showed that the expression levels of TNF-α, IL-6 and IL-1β significantly decreased in breast tissue of the SMPs treatment group. Western Blot results showed that TLR4, MyD88 and NF-κB protein levels significantly decreased in SMPs treatment group. RT-qPCR results further showed the significant reduces of the mRNA transcription levels of TNF-α, IL-6 and IL-1β in the SMPs treatment group. The results showed that SMPs had a protective effect on LPS-induced mammary inflammation, and its anti-inflammatory effect was related to the inhibition of the expression of key proteins in the TLR4 signaling pathway.更多还原