【摘要】 以金黄色葡萄球菌作为试验菌，3 cm×3 cm的JB-00棉布作为染菌载体，8种市售织物洗涤剂作为待测试样，使用无菌硬水将待测试样于广口瓶中稀释至作用浓度后，向每个广口瓶中加入3片染菌载体后置于200 r/min磁力搅拌下洗涤20 min，再换水漂洗2次，每次5 min，以模拟织物实际的洗涤过程，最后通过平板计数法计算不同产品的除菌率。结果表明，试样A,G,H的除菌率在95%以上，试样B的除菌率在80%以上，试样C和E的除菌率在60%以上，试样D的除菌率在30%以上，试样F的除菌率在30%以下。可见，使用该方法对不同试样的除菌性能具有较好的区分度（P<0.05）；使用该方法评价除菌性能的稳定性与试样除菌率的高低有关，对于除菌效果好的试样A,G,H的除菌率的稳定性也较高。因此，该方法可作为织物洗涤剂除菌性能的测试评价方法。更多还原

【Abstract】 Staphylococcus aureus was used as the test bacteria, and the 3 cm×3 cm JB-00 cotton cloth was used as the carrier. The staining carrier was prepared by inoculating 20 μL of the test bacteria suspension on each piece of cotton cloth by the 5-point method. 8 kinds of commercially available fabric detergents were used as the samples to be tested. Sterile hard water was used as the diluent to dilute the sample to be tested in a wide-mouth bottle to an effective concentration. 3 pieces of staining carrier were added to each wide-mouth bottle and washed for 20 minutes with magnetic stirring at 200 r/min, and then the water was changed and they were rinsed for 2 times and 5 min each time, which simulated the actual washing process of the fabric. Finally, the sterilization rate of different products was calculated by the plate counting method. The results show that the sterilization rate of samples A, G and H is above 95%, the sterilization rate of sample B is above 80%, the sterilization rate of samples C and E is above 60%, the sterilization rate of sample D is above 30%, and the sterilization rate of sample F is below 30%. This method can effectively distinguish the sterilization performance of different samples( P<0.05); The stability of sterilization performance evaluated by this method is related to the level of the sterilization rate of the sample. For samples A, G and H that have good sterilization effects, the stability of sterilization rate is also high, while for samples D and F that have poor sterilization effects, the stability of sterilization rate is also low. Therefore, this method can be used as an evaluation method for the sterilization performance of fabric detergents.更多还原