【摘要】 目的 探讨膜联蛋白A7(ANXA7)低表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法 将人胃癌MGC-803细胞分为ANXA7低表达组(si-ANXA7)和阴性对照组(si-con)，分别将靶向ANXA7的si-RNA和阴性对照RNA转染为ANXA7低表达组和阴性对照组。Western blot法检测ANXA7的表达；MTT法检测各组细胞的增殖能力；PI检测各组细胞的凋亡率；Western blot检测各组细胞增殖和凋亡相关蛋白PCNA和Cleaved casepase-3的表达。结果 与阴性对照组相比，ANXA7低表达组MGC-803细胞增殖能力显著降低，增殖相关蛋白PCNA表达显著下降(P<0.05)；细胞凋亡增强，相关蛋白Cleaved casepase-3的表达显著升高(P<0.05)。结论 ANXA7低表达可抑制人胃癌MGC-803细胞的增殖并促进其凋亡。更多还原

【Abstract】 Objective To investigate the effects of low-expression of annexin A7 (ANXA7) on the proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods Human gastric cancer MGC-803 cells were divided into ANXA7 low-expression group (si-ANXA7),negative control group (si-con).Si-RNA targeting ANXA7 and negative control RNA were transfected into ANXA7 low-expression group and negative control group respectively.The expression of ANXA7 was detected by Western blot;MTT assay was used to detect the proliferation of cells in each group;The apoptosis rate in each group was detected by PI;The expressions of PCNA and Cleaved casepase-3 were detected in each group by Western blot.Results Compared with the negative control group,the proliferation ability of MGC-803 cells in the low-expression group of ANXA7 decreased significantly,and the expression of proliferation related proteins PCNA decreased significantly (P<0.05);The expression of cleaved casepase-3 increased significantly (P<0.05).Conclusion The low-expression of ANXA7 may inhibitthe proliferation and promote the apoptosis of human gastric cancer MGC-803 cells.更多还原