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Box-Behnken法优化酸枣仁总皂苷和多糖提取工艺及抗氧化作用研究
Study on optimization of the extraction process of total saponins and polysaccharides from semen ziziphi spinosae by Box-Behnken method and its antioxidant effect
【摘要】 为优化酸枣仁总皂苷和多糖提取工艺,及研究其抗氧化作用,采用Box-Behnken法优化提取工艺,利用3种抗氧化检测方法对酸枣仁不同炮制品进行抗氧化作用研究。试验结果表明,酸枣仁总皂苷最佳提取工艺为超声功率228.5 W,液固比70:1,乙醇体积分数10%,提取时间21.1 min;多糖最佳提取工艺:超声功率200 W,液固比70:1,乙醇体积分数21.2%,提取时间27.7 min;炒酸枣仁总皂苷含量较高,生-炒配伍的多糖含量较高。研究表明酸枣仁的总皂苷和多糖均具有一定抗氧化能力,优化的工艺可为酸枣仁的开发和应用提供依据。
【Abstract】 To optimize the extraction process of total saponins and polysaccharides of the jujube kernel and study its antioxidant effect,Box-behnken method was used to optimize the extraction process of total saponins and polysaccharides from semen ziziphi spinosae,and three antioxidant detection methods were used to study the antioxidant effects of different processed products of semen ziziphi spinosae.The results show that optimal extraction conditions of total saponins are as follows:ultrasonic power 228.5 W,liquid-solid ratio 70:1,ethanol volume fraction 10%,and extraction time 21.1 min.The best extraction process of polysaccharides:ultrasonic power 200 W,liquid-solid ratio 70:1,ethanol volume fraction 21.2%,and extraction time 27.7 min.The total saponin content of stir-fried semen ziziphi spinosae is higher,and the polysaccharide content of raw-stir-fried semen ziziphi spinosae is higher.The total saponins and polysaccharides of semen ziziphi spinosae have certain antioxidant capacity.The optimized process can provide a basis for the development and application of jujube kernel.
【Key words】 semen ziziphi spinosae; total saponins; polysaccharides; extraction process optimization; antioxidation;
- 【文献出处】 包装与食品机械 ,Packaging and Food Machinery , 编辑部邮箱 ,2022年06期
- 【分类号】TS201.2
- 【下载频次】63