【摘要】 目的：采用正交试验设计，优选升陷汤最佳醇提工艺。方法：建立高效液相色谱-二极管阵列检测器-蒸发光检测器联用技术（HPLC-DAD-ELSD）方法同时测定芒果苷、毛蕊异黄酮苷及知母皂苷BⅡ，建立HPLC-ELSD方法测定黄芪甲苷的含量。对上述两种分析方法均进行了方法学考察。以芒果苷、毛蕊异黄酮苷、知母皂苷BⅡ、黄芪甲苷的转移率及干膏率为指标，采用SPSS一般线性模型分析方法考察乙醇浓度、加醇倍数和提取时间3个因素，采用正交试验法对升陷汤的制备工艺进行优化。结果：工艺筛选结果表明，升陷汤最佳醇提工艺为黄芪、知母、桔梗、柴胡和升麻加10倍量的70%乙醇回流2.5 h。结论：优选的工艺稳定、合理，为升陷汤的后续制备工艺及药理研究提供了科学依据。更多还原

【Abstract】 Objective: To optimize the ethanol extraction procession of Shengxian Decoction by orthogonal design.Methods: The HPLC-DAD-ELSD method was established for the simultaneous determination of mangiferin, verbascoside,and timosaponin B Ⅱ, and the HPLC-ELSD method was established to assay the astragaloside IV. The two analytical methods mentioned above are investigated by methodology. SPSS general linear model method was used to study the three factors including the ethanol concentration, the ratio of extracting medium, and the extracting time, by taking the dry extract yield of the medicinal materials and contents of mangiferin, verbascoside, timosaponin B I, and astragaloside IV as indicators, the orthogonal design was used to optimize the ethanol extraction procession of Shengxian Decoction. Results: The established HPLC-DADELSD and HPLC-ELSD methods were stable and feasible and could be used for quality control of Shengxian Decoction. Besides,it was determined that the optimal ethanol extraction procession of Shengxian Decoction was that Astragali Radix, Anemarrhenae Rhizoma, Platycodonis Radix, Bupleuri Radix and Cimicifugae Rhizoma were extracted with 10 times of 70% ethanol, refluxed for 2.5 h. Conclusion: The optimal process is stable and reasonable for the ethanol extraction of Shengxian Decoction, which provides scientific data for its standardized production in the future.更多还原