【摘要】 目的：对桂枝加葛根汤（GGD）提取物进行化学成分表征和体内抗流感病毒活性初步研究。方法：采用Halo-C18色谱柱，以0.5%甲酸溶液-0.5%甲酸乙腈溶液为流动相梯度洗脱；通过质谱正负离子模式对提取物中化学成分进行检测。建立小鼠H1N1流感模型，以奥司他韦（达菲）为阳性对照药，GGD进行干预，测定小鼠肺指数，利用RT-qPCR技术测定小鼠肺部病毒载量及肺组织中SP-A、SP-D mRNA相对表达量，利用HE染色观察肺组织的病理状态。结果：通过UPLC-Q-Exactive/MS初步分析鉴定了GGD中71种化合物。与模型组比较，GGD可显著降低流感小鼠的肺指数及肺部病毒载量（P<0.01,P<0.05），升高肺部SP-A、SP-DmRNA相对表达量（P<0.01,P<0.05），改善流感病毒所致的肺部炎症损伤。结论：UPLC-Q-Exactive/MS可快速准确鉴定出GGD中化学成分，且GGD具有能较好抗H1N1流感病毒的活性，该研究为GGD后续药效学及药效物质基础研究提供重要的理论基础。更多还原

【Abstract】 Objective: To analyze the chemical components of Guizhi Gegen Decoction(GGD), and preliminarily explored its antiviral activity in vivo. Methods: Halo-C18 column was used for chromatographic elution by gradient elution with 0.5% formic acid solution-0.5% formic acid acetonitrile solution. The ESI was used for mass spectrometry with negative and positive monitoring modes. Mice were inoculated with IAV H1N1 and then treated with GGD and oseltamivir phosphate, the lung index and viral load of mice were measured, and the relative expression of SP-A and SP-D in lung tissue were measured by RTqPCR. Observation of pathological state of lung tissue by HE staining. Results: A total of 71 compounds were inferred by UPLCQ-Exactive/MS. Compared with the model group, GGD treatment reduced the viral load of lung tissue and lung index(P<0.01,P<0.05), improved the relative expression of SP-A and SP-D(P<0.01, P<0.05), and improved the lung inflammatory damage caused by influenza virus. Conclusion: UPLC-Q-Exactive/MS can quickly and accurately identify the chemical constituents of GGD, and GGD has good antiviral activity, which provides important theoretical guidance for the follow-up pharmacodynamics and pharmacodynamic material basis research of GGD.更多还原