【摘要】 目的：研究柚皮苷（NG）对Aβ_25-35损伤PC12细胞中ER-P38/MAPK信号通路的调控效应。方法：以NG为受试药物，使用Aβ_25-35损伤PC12细胞建立阿尔茨海默症（AD）细胞模型，MTT法检测细胞增殖率，Western Blot法检测各组在阻断ER与P38-MAPK信号通路前后ERβ、p-P38/P38、Bcl-2、Caspase-3蛋白表达量的变化，探究NG对Aβ_25-35致PC12细胞损伤的保护作用。结果：与模型组比较，NG+Aβ_25-35组可引起受试PC12细胞中ERβ、Bcl-2蛋白表达显著升高以及p-P38/P38、Caspase-3表达显著降低（P<0.01）；阻断ER后，与NG+Aβ_25-35组比较，NG+Aβ_25-35+ICI182780组ERβ和Bcl-2的表达显著降低（P<0.01）,p-P38/P38和Caspase-3表达升高（P<0.01）；阻断P38-MAPK通路后，与NG+Aβ_25-35组比较，NG+Aβ_25-35+SB203580组Bcl-2的表达降低（P<0.01）,p-P38/P38和Caspase-3表达升高（P<0.01）。结论：NG可通过对ER-P38/MAPK途径的调控，减少受损PC12细胞的细胞凋亡，NG可作为神经保护剂进行深入开发。更多还原

【Abstract】 Objective: To study the regulating effect of naringin(NG) on the ER-P38/MAPK signaling pathway in PC12cells damaged by Aβ25-35. Methods: With NG as the test drug, Aβ25-35 was used to damage PC12 cells to establish an Alzheimer’s disease(AD) cell model. MTT method was used to detect cell proliferation rate, and Western Blot method was used to detect ERβand p-P38/P38, Bcl-2, Caspase-3 protein expression changes, to explore the protective effect of NG on Aβ25-35 induced PC12 cell injury. Results: Compared with the model group, the NG+Aβ25-35 group could significantly increase the protein expressions of ERβand Bcl-2 and significantly decrease the expressions of p-P38/P38 and Caspase-3 in the PC12 cells tested(P<0.01); blocking ER compared with the NG+Aβ25-35 group, the NG+Aβ25-35+ICI182780 group could significantly reduce the expression of ERβ and Bcl-2(P<0.01), and increase the expression of p-P38/P38 and Caspase-3(P<0.01); after blocking the P38-MAPK pathway, compared with the NG+Aβ25-35 group, the NG+Aβ25-35+SB203580 group could reduce the expression of Bcl-2(P<0.01), and increase p-P38/P38 and Caspase-3 expression(P<0.01). Conclusion: NG can reduce the apoptosis of damaged PC12 cells by regulating the ER-P38/MAPK pathway. NG can be further developed as a neuroprotective agent.更多还原