【摘要】 目的：探讨逍遥散对慢性束缚应激（CRS）大肠癌裸鼠肠道菌群代谢产物的影响。方法：40只SPF级雄性BALB/c裸鼠随机分为正常组、模型组、逍遥散组、氟西汀组，每组10只。除正常组外，其余3组裸鼠均接受CRS造模，持续2周。CRS造模结束后1周，4组裸鼠全部皮下注射HCT116细胞，建立CRS大肠癌皮下移植瘤模型，逍遥散组和氟西汀组分别给予逍遥散和氟西汀干预。灌胃3周后，无菌条件下收集4组裸鼠粪便，采用LC-MS技术检测肠道菌群代谢产物的种类和含量。结果：4组间共检测出差异代谢物20种，与正常组比较，模型组裸鼠肠道菌群代谢产物中LysoPE(15∶0/0∶0)、PE(16∶0/0∶0)、Oleamide、DL-Phenylalanine和2-Hydroxyphenethylamine的含量均显著减少（P<0.01,P<0.05）；与模型组比较，逍遥散组和氟西汀组裸鼠肠道菌群代谢产物中LysoPE(15∶0/0∶0)、PE(16∶0/0∶0)和Oleamide含量均显著增加（P<0.01,P<0.05）。结论：逍遥散可能通过增加肠道菌群代谢产物中LysoPE(15∶0/0∶0)、PE(16∶0/0∶0)和Oleamide含量，从而达到改善CRS裸鼠抑郁状态和抑制CRS大肠癌皮下移植瘤生长的作用。更多还原

【Abstract】 Objective: To investigate the effects of Xiaoyao Powder on the metabolites of gut microbiota in nude mice with chronic restrained stress(CRS) colorectal cancer. Methods: A total of 40 SPF male BALB/c nude mice were randomly divided into normal group, model group, Xiaoyao Powder group, fluoxetine group with 10 nude mice in each group. Except for the normal group,the other 3 groups of nude mice all received CRS modeling for 2 weeks. One week after the completion of CRS modeling, all the 4 groups of nude mice were injected subcutaneously with HCT116 cells to establish subcutaneous transplantation models of CRS colorectal cancer. The groups of Xiaoyao Powder and fluoxetine were treated with Xiaoyao Powder and fluoxetine respectively. After 3 weeks of intragastric administration, the feces of the 4 groups were collected under aseptic conditions, and the types and contents of metabolites of gut microbiota were detected by liquid chromatograph-mass spectrometer. Results: A total of 20 different metabolites were detected among the 4 groups. Compared with the normal group, the content of LysoPE(15∶0/0∶0), PE(16∶0/0∶0),Oleamide, DL-Phenylalanine and 2-Hydroxyphenethylamine in the model group were all decreased significantly(P<0.01, P<0.05).Compared with the model group, the content of LysoPE(15∶0/0∶0), PE(16∶0/0∶0) and Oleamide in the Xiaoyao Powder group and fluoxetine group were all increased significantly(P<0.01, P<0.05). Conclusion: Xiaoyao Powder may increase the content of Lyso PE(15∶0/0∶0), PE(16∶0/0∶0) and Oleamide in the metabolites of gut microbiota, thereby improving the depression of nude mice under CRS and inhibiting the growth of subcutaneously transplanted tumors of CRS colorectal cancer.更多还原