【摘要】 目的:探讨目的基因DKK3及CDKN2A在急性髓系白血病患者中各时期的甲基化水平，以及DNA甲基化在急性髓系白血病患者发生及发展的作用。方法:采用甲基化特异性PCR(MSP)检测例AML患者初发时，完全缓解时及复发时DKK3,CDKN2A基因甲基化水平。另追踪16例AML患者各时期DKK3,CDKN2A基因甲基化水平。结果:对照组中DKK3及CDKN2A基因甲基化检出率分别为8.3%(1/12),0%(0/12);初发组50例AML患者中DKK3及CDKN2A基因甲基化检出率分别为88.0%(44/50),58.0%(29/50),与对照组比较具有统计学差异(P<0.001)。缓解组31例AML患者中DKK3及CDKN2A基因甲基化检出率分别为25.8%(8/31),9.7%(3/31),与对照组比较无统计学差异(P>0.05)。复发组16例AML患者中DKK3及CDKN2A基因甲基化检出率分别为75.0%(12/16),56.3%(9/16),与对照组比较具有统计学差异(P<0.001)。在各FAB分型中，DKK3及CDKN2A基因甲基化检出率均无统计学差异(P>0.05)。追踪16例患者中，DKK3基因甲基化初发组与缓解组具有统计学差异(P<0.0001),缓解组与复发组间具有统计学差异(P<0.0001),缓解组与复发组间具有统计学差异(P<0.05),初发组与复发组间无统计学差异(P>0.05)。CDKN2A基因相对表达量，初发组与缓解组存在统计学差异(P<0.0001),初发组与复发组存在统计学差异(P<0.05)。结论:DKK3、CDKN2A基因甲基化在急性髓系白血病患者的发展过程及预后监测中具有重要的临床意义。更多还原

【Abstract】 Objective:To investigate the methylation levels of DKK3 and CDKN2 A in patients with acute myeloid leukemia at different stages, and the role of DNA methylation in the occurrence and development of acute myeloid leukemia.Methods:Methylation specific PCR(MSP) was used to detect the methylation levels of DKK3 and CDKN2 A genes in AML patients at the onset, complete remission and recurrence.The methylation levels of DKK3 and CDKN2 A genes in 16 AML patients at different stages were also tracked.Results:The methylation detection rates of DKK3 and CDKN2 A genes in the control group were 8.3 %(1/12) and 0 %(0/12), respectively.The methylation detection rates of DKK3 and CDKN2 A genes in 50 AML patients in the primary group were 88.0 %(44/50) and 58.0 %(29/50), respectively, which were significantly different from those in the control group(P<0.05).The methylation detection rates of DKK3 and CDKN2 A genes in 31 AML patients in the remission group were 25.8 %(8/31) and 9.7 %(3/31), respectively, which were not significantly different from those in the control group(P>0.05).The methylation detection rates of DKK3 and CDKN2 A genes in 16 AML patients in the recurrence group were 75.0 %(12/16) and 56.3 %(9/16), respectively, which were statistically different from those in the control group(P<0.001).In each FAB typing, there was no significant difference in the methylation detection rates of DKK3 and CDKN2 A genes(P>0.05).Among the 16 patients, the relative expression of DKK3 gene in the primary group and the remission group were statistically different(P<0.05); the relative expression of DKK3 in the remission group and the recurrence group was statistically different(P<0.05); there was no significant difference in the relative expression of DKK3 between the primary group and the recurrence group(P>0.05).The relative expression of CDKN2 A gene in primary group and the remission group were statistically different(P<0.0001); the relative expression of CDKN2 A gene in the remission group and the recurrence group were statistically different(P<0.05); the relative expression of CDKN2 A gene in the primary group and the recurrence group were statistically different(P<0.05).Conclusion:The methylation of DKK3 and CDKN2 A genes has important clinical significance in the development process and prognosis monitoring of patients with acute myeloid leukemia.更多还原