【摘要】 目的 探讨转化生长因子β诱导基因（TGFβI）在胃癌中的表达及与幽门螺杆菌（Hp）感染的关系。方法 取50例胃癌患者的胃癌组织，取52例慢性萎缩性胃炎（CAG）患者及54例慢性浅表性胃炎（CSG）患者的胃黏膜组织，采用定量聚合酶链反应（qPCR）检测TGFβI mRNA的相对表达量，分析TGFβI与Hp感染及患者临床特征的关系。结果 胃癌组织中TGFβI mRNA相对表达量高于CAG组织和CSG组织，差异均有统计学意义（P﹤0.05）。采用¹³C呼气实验来判定Hp的感染状态，Hp阳性患者中，胃癌组织中TGFβI mRNA相对表达量高于CAG组织和CSG组织，胃癌组织、CAG组织和CSG组织中TGFβI mRNA相对表达量均高于Hp阴性患者，差异均有统计学意义（P﹤0.05）。中低分化、浸润深度为T_3～4、TNM分期为Ⅲ～Ⅳ期胃癌患者胃癌组织中TGFβI mRNA的相对表达量分别高于高分化、浸润深度为T_1～2、TNM分期为Ⅰ～Ⅱ期的患者，差异均有统计学意义（P﹤0.05）。结论 胃癌组织中TGFβI高表达，且其表达可能与胃癌患者分化程度、浸润深度、TNM分期有关，Hp感染可能通过一定的机制促进TGFβI的表达，进而促进胃癌的发生发展过程。更多还原

【Abstract】 Objective To investigate the expression of transforming growth factor β induced gene(TGFβI) in gastric cancer and its relationship with helicobacter pylori(Hp) infection. Method Gastric cancer tissues from 50 patients with gastric cancer, gastric mucosal tissues from 52 patients with chronic atrophic gastritis(CAG) and 54 patients with chronic superficial gastritis(CSG) were collected. The relative expression of TGFβI mRNA was detected by quantitative polymerase chain reaction(qPCR). The relationship between TGFβI and Hp infection and clinical characteristics of patients were also analyzed. Result The relative expression of TGFβI mRNA in gastric cancer tissue was significantly higher than that in CAG tissue and CSG tissue, and the differences were statistically significant(P<0.05). The13C breath test was used to determine the infection status of Hp. In Hp-positive patients, the relative expression of TGFβI mRNA in gastric cancer tissue was significantly higher than that in CAG tissue and CSG tissue, and the relative expression of TGFβI mRNA in gastric cancer tissue, CAG tissue, and CSG tissue were higher than those in Hp-negative patients, and the differences were statistically significant(P<0.05). The relative expression of TGFβI mRNA in gastric cancer tissues of patients with moderately-to-poorly differentiated, T3-4infiltration depth and TNM stage III-IV were higher than those of well-differentiated, T1-2infiltration depth and TNM stage I-II, respectively, the differences were statistically significant(P<0.05).Conclusion TGFβI is highly expressed in gastric cancer tissue, and its expression may be related to the degree of differentiation, depth of invasion, and TNM stage of gastric cancer patients. Hp infection may promote the expression of TGFβI through a certain mechanism, thereby promoting the occurrence and development of gastric cancer.更多还原