【摘要】 为探讨不同浓度的硒溶液对小麦抗倒春寒能力的影响，以盆栽低温敏感型小麦品种郑麦366为试验材料，分别用0、0.3、0.6、0.9、1.2、1.5、2.4 mg/L的亚硒酸钠溶液喷施雌雄蕊原基分化期的郑麦366幼苗，3 d后将各处理材料放入人工气候室0℃低温胁迫处理36 h,测定各处理中郑麦366叶片的抗氧化酶活性及丙二醛含量，成熟期调查结实性。结果表明，36 h低温处理显著降低了郑麦366的结实率，不同浓度亚硒酸钠溶液处理后低温胁迫的郑麦366结实性和叶片抗氧化酶活性均呈现先升高再降低的趋势。经亚硒酸钠溶液喷施后低温处理的结实性均低于常温对照处理，0.9 mg/L的亚硒酸钠溶液处理增加了郑麦366叶片中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)的活性，结实性最高；丙二醛(MDA)含量呈现出先降低再升高的趋势，在0.9 mg/L的处理浓度下含量最低。结果表明，硒通过提高郑麦366叶片中抗氧化酶活性、降低丙二醛含量来提高小麦应对倒春寒胁迫的能力，该研究结果为硒用于小麦抗倒春寒胁迫的研究提供了理论参考，为提高大田小麦倒春寒能力提供技术支撑。更多还原

【Abstract】 In order to investigate the effects of different concentrations of selenium solution on the resistance of wheat to reverse spring cold, the potted low-temperature sensitive wheat variety Zhengmai 366 was used as experimental material. Zhengmai 366 seedlings were sprayed with 0, 0.3, 0.6, 0.9, 1.2, 1.5 and 2.4 mg/L sodium selenite solutions, respectively. After 3 days, each treatment material was placed into an artificial climate chamber under low temperature stress at 0℃ for 36 h.The antioxidant enzyme activities and malondialdehyde content of zhengmai 366 leaves were determined in each treatment, and fruit setting was investigated at maturity stage.The results showed that low temperature treatment for 36 h significantly reduced the seed setting rate of Zhengmai 366, and the seed setting and antioxidant enzyme activity of Zhengmai 366 under different concentrations of sodium selenite solution increased first and then decreased. After spraying with sodium selenite solution, the seed setting of low temperature treatment was lower than that of normal temperature control. 0.9 mg/L sodium selenite solution increased the activities of catalase(CAT), superoxide dismutase(SOD), peroxidase(POD) and ascorbate peroxidase(APX) in Zhengmai 366 leaves, and the seed setting was the highest. The content of malondialdehyde(MDA) showed a trend of first decreasing and then increasing, and the content was the lowest at 0.9 mg/L. In conclusion, selenium could improve the ability of wheat to cope with inverted cold stress by increasing the activity of antioxidant enzymes and reducing the content of malondialdehyde in Zhengmai 366 leaves. The results provided theoretical reference for the study of selenium in wheat resistance to inverted cold stress, and provided technical support for improving the ability of wheat to cope with inverted cold stress in field.更多还原