【摘要】 [目的]研究微波辅助酸解在低分子量海带褐藻糖胶制备中的可行性。[方法]采用酸提取法制备海带褐藻糖胶，测定在不同HCl浓度(0、1、2 mol/L)、不同降解时间(40、50、60 min)、不同降解温度(70、80、90℃)条件下微波辅助酸解得到的褐藻糖胶的分子量，分析不同降解条件对褐藻糖胶分子量的影响，并与水浴辅助酸解的结果进行对比分析。[结果]随着HCl浓度、降解时间、降解温度的升高，酸解产物的分子量降低，且温度对降解产物分子量的影响最大。与水浴加热相比，微波辅助酸解降解产物的分子量更低。在80℃,HCl浓度为1 mol/L,降解40 min以上，比较容易制备低分子量褐藻糖胶。微波比水浴加热更容易对褐藻糖胶的硫酸基造成破坏。在2 mol/L HCl条件下，微波和水浴辅助酸解60 min,分别在70和90℃观察到明显的硫酸基脱除现象。[结论]采用微波辅助酸解法制备低分子量褐藻糖胶时，要注意硫酸基脱除的问题。更多还原

【Abstract】 [Objective] To study the feasibility of microwave-assisted acidolysis in the preparation of low molecular weight kelp fucoidan. [Method]Kelp fucoidan was prepared by acid extraction. The molecular weight of fucoidan obtained by microwave-assisted acidolysis under different HCl concentration(0, 1, 2 mol/L), different degradation time(40, 50, 60 min) and different degradation temperature(70, 80, 90 ℃), was measured. The effects of different degradation conditions on the molecular weight of fucoidan were analyzed and compared with the results of water bath assisted acidolysis. [Result]With the increase of HCl concentration, degradation time and degradation temperature, the molecular weight of acid hydrolysis products decreased, and the temperature had the greatest influence on the molecular weight of degradation products.Compared with water bath heating, the molecular weight of microwave-assisted acid hydrolysis degradation products was lower. It was easy to prepare low molecular weight fucoidan at 80 ℃, HCl concentration of 1 mol/L and degradation for more than 40 min. Microwave was easier to destroy the sulfate group of fucoidan than water bath heating. Under the condition of 2 mol/L HCl, microwave and water bath assisted acidolysis for 60 min, obvious sulfate removal was observed at 70 ℃and 90 ℃ respectively. [Conclusion]When preparing low molecular weight fucoidan by microwave-assisted acidolysis, attention should be paid to the removal of sulfate group.更多还原