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水稻叶褐条斑病病原菌的分离鉴定与接种技术研究
Isolation,Identification and Inoculation Techniques of Pathogenic Bacteria from Brown Leaf Streak in Rice
【摘要】 在黑龙江稻区采集病样、分离病原菌的基础上,获得形态差异明显的20株水稻叶褐条斑病菌,利用ITS序列鉴定其均为Nigrospora oryzae,由此推断Nigrospora oryzae为当地主要的水稻黑孢菌种群。此外,依据水稻叶褐条斑病的生物学特征,摸索了人工接种鉴定技术。该研究结果为抗水稻叶褐条斑病的遗传育种研究奠定基础。
【Abstract】 In this study, 20 strains of BLS disease were isolated and identified from rice as Nigrospora oryzae by internal transcribed spacer(ITS) sequence analysis.It was concluded that Nigrospora oryzae was the main population of rice melanospora in this area.In addition, we have explored the artificial inoculation and identification technique according to the biological characteristics of LBS in rice.The results of this study laid a foundation for the program of genetic breeding of resistance to BLS in rice.
【关键词】 稻黑孢菌;
Nigrospora oryzae;
分离与鉴定;
标准化接种技术;
【Key words】 Rice black fungus; Nigrospora oryzae; Isolation and identification; Standardized inoculation technique;
【Key words】 Rice black fungus; Nigrospora oryzae; Isolation and identification; Standardized inoculation technique;
【基金】 黑龙江省自然科学基金(YQ2020C040);黑龙江省博士后基金(LBH-Z19207);黑龙江省农业科技创新工程项目(2018YYYF004,HNK2019CX14);黑龙江省省属科研院所科研业务费(CZKYF2021B005);黑龙江省农业科学院长期性基础性项目(2019CQJC002,2020CQJC002,2021CQJC002)
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2022年06期
- 【分类号】S435.111.4
- 【下载频次】252