【摘要】 [目的]研究植物提取物姜辣素及其与牛磺酸的复合制剂对高温下加州鲈鱼抗氧化应激能力的影响。[方法]当水温为30～33℃时，采用3.0 mg/L的姜辣素及其与牛磺酸的复合制剂水体泼洒，每2 d使用1次，连续10 d;按0.1%的添加量将其添加到加州鲈鱼饲料中，每天投喂2次，连续喂养20 d。采集加州鲈鱼肌肉和肝脏组织样本，采用比色法测定其肝脏和肌肉中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽-过氧化物酶(GSH-Px)的活性以及丙二醛(MDA)含量。[结果]当水温为30～33℃时，与10 d相比，加州鲈鱼养殖20 d时其肌肉和肝脏组织中的T-AOC以及SOD、CAT和GSH-Px活性有所下降，而MDA含量有所上升。采用姜辣素、姜辣素+牛磺酸复合制剂水体泼洒10 d后，加州鲈鱼肌肉和肝脏组织中的T-AOC以及SOD、CAT和GSH-Px活性有所上升，而MDA含量有所下降。饲料投喂20 d后，加州鲈鱼肌肉和肝脏组织中的抗氧化应激指标均发生了显著变化(P<0.05),T-AOC以及SOD、CAT和GSH-Px活性显著上升，MDA含量则显著下降。[结论]水体泼洒或饲料投喂姜辣素及姜辣素+牛磺酸复合制剂均可以有效提高加州鲈鱼的抗氧化应激能力，饲料投喂方式较水体泼洒方式更加有效，姜辣素+牛磺酸复合制剂比姜辣素的作用效果更加显著。更多还原

【Abstract】 [Objective] To study the effects of plant extract gingerol and its compound preparation with taurine on the antioxidant stress ability of Micropterus salmoides under high temperatures.[Method] When the water temperature was 30-33 ℃,plant extract gingerol and its compound preparation with taurine were sprinkled into water at the concentration of 3.0 mg/L,they were used once every two days for 10 days.In another treatment, plant extract gingerol and its compound preparation with taurine was added in the feed of M.salmoides with the addition amount of 0.1%.M.salmoides were fed twice every day for 20 days.The muscle and liver tissue samples of M.salmoides were collected to determine total antioxidant capacity(T-AOC),the activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GSH-Px),malondialdehyde(MDA) content in the liver and muscles by using colorimetric method. [Result]When the water temperature was 30-33 ℃,compared with 10 days, T-AOC and the activities of SOD,CAT,GSH-Px in the muscles and liver of M.salmoides cultured for 20 days decreased, while the content of MDA increased.After 10 days of water sprinkling with gingerol, gingerol compound with taurine, T-AOC and the activities of SOD,CAT and GSH-Px in the liver and muscle tissue of M.salmoides all showed upward trends, while MDA content showed a downward trend.After continuous feeding for 20 days, the antioxidant stress indices in the liver and muscle tissues of M.salmoides changed significantly(P< 0.05),T-AOC and the activities of SOD,CAT and GSH-Px increased significantly, while MDA content decreased significantly.[Conclusion]Water sprinkling or feeding with gingerol and gingerol-taurine compound preparation could effectively improve the antioxidant stress capacity of M.salmoides.Continuous feeding was more effective than water sprinkling, and gingerol-taurine compound preparation had more significant effects than single gingerol.更多还原