【摘要】 [目的]通过研究不同品种杂交高粱抗性生理指标的差异并探索其可溶性糖的含量与SS酶及SPS酶活性的关系,建立高效精确选育抗性强产量高的优质高粱种质资源的重要途径。[方法]选用愈创木酚比色法、氮蓝四唑光还原法、紫外分光光度计法分别提取并测定POD、SOD、CAT活性;采用优化后的蒽酮比色法测定叶片可溶性糖含量并以硫代巴比妥酸法测定MDA含量;SS及SPS活性的测定均采用间苯二酚比色法。[结果]辽糯10号同时具有抗逆性强、可溶性糖含量高的双重优势;辽糯15号SOD和CAT活性最高但MDA含量最低,表明其抗逆性在所有品种中最强;辽杂66号具有较高的SS及SPS活性,从而对糖分积累有促进作用,利于实现高产。[结论]从多角度阐明了高粱抗旱、耐盐碱机制,丰富其抗性生理内容;进一步发掘并了解高粱糖分积累机制,明确高粱糖分积累分子调节机制的内在机理,建立了精确选育抗性强、产量高的优质高粱种质资源的重要途径,并制定强化高粱抗性及产量的技术措施,为筛选近缘农作物的优质种质资源、提高农作物的育种技术水平、筛选其差异表达基因后再进行蛋白质组和转录组关联分析、挖掘调控高粱抗性强弱及糖分积累的关键基因及解析其分子调控机制奠定坚实的理论基础。更多还原

【Abstract】 [Objective] To explore the relationship between soluble sugar content and SS and SPS enzyme activities in sorghum, and to establish an effective way to select high-quality sorghum germplasm resources with strong resistance and high yield. [Method] The activities of POD, SOD and CAT were extracted and determined by guaiacol colorimetry, azoblue tetrazole photoreduction and UV spectrophotometer, respectively. The contents of total available sugar and acetaldehyde were determined by antraton non-color optimization method and mercaptan acid non color method, respectively. The activities of SS and SPS were determined by isophthalic phenol colorimetry. [Result] Liaonuo 10 had the double advantages of strong stress resistance and high soluble sugar content at the same time; Liaonuo 15 had the highest SOD activity, CAT activity and the lowest MDA content, which indicated that it had the strongest stress resistance; Liaoza 66 had the highest SS and SPS enzyme activities, which promoted sugar accumulation and achieved high yield. [Conclusion] The mechanism of drought resistance and saline alkali tolerance of sorghum was revealed from many aspects, and the physiological content of stress resistance of sorghum was enriched; the mechanism of sugar accumulation in sorghum was explored and further understood, and the internal mechanism of molecular regulation mechanism of sugar accumulation in sorghum was clarified. An important way to accurately select high-quality sorghum germplasm resources with strong resistance and high yield was established, and technical measures to strengthen sorghum resistance and yield were formulated, so as to screen the high-quality germplasm resources of related crops, to enhance crop breeding level, to screen different expressed genes, to carry out proteome and transcriptome association analysis, to explore the regulation of sorghum resistance, and to find out the key genes of sexual strength and sugar accumulation and their molecular regulation mechanism.更多还原