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PM_2.5混悬液对3种癌细胞侵袭和迁移能力的影响

Effects of PM_2.5 on invasion and migration abilities in three types of cancer cells

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【Author】 LI Boru;QIN Shuangjian;CAI Ying;LI Runbing;GUAN Lan;XIAO Fang;ZENG Ming;XU Xinyun;Xiangya School of Public Health, Central South University;Shenzhen Center for Disease Control and Prevention;School of Public Health, University of South China;

【通讯作者】徐新云;

【机构】中南大学湘雅公共卫生学院；深圳市疾病预防控制中心；南华大学公共卫生学院；

【摘要】目的：探讨PM_2.5混悬液对人肺癌A549细胞、人肝癌HepG2细胞和人肾癌A498细胞迁移和侵袭能力的影响。方法：采用10、50μg/mL的PM_2.5混悬液分别对A549、HepG2和A498细胞染毒24 h,Transwell小室实验检测细胞迁移、侵袭能力。结果：迁移实验结果显示，阴性对照组、10和50μg/mL PM_2.5染毒组A549细胞穿膜细胞数分别为（356.33±30.92）、（476.33±28.50）和（563.33±74.00）个；HepG2细胞分别为（86.42±4.75）、（140.00±16.15）和（234.17±9.06）个；A498细胞分别为（42.33±6.43）、（80.00±6.08）和（174.67±25.01）个。侵袭实验结果显示，阴性对照组、10和50μg/mL PM_2.5染毒组A549细胞的穿膜细胞数分别为（188.33±9.45）、（313.00±7.94）和（404.00±57.66）个；HepG2细胞分别为（48.33±3.26）、（97.75±6.63）和（123.92±8.57）个；A498细胞分别为（5.67±5.03）、（21.33±5.13）和（40.67±6.81）个。在迁移实验和侵袭实验中，与阴性对照组相比，PM_2.5染毒组中的3种细胞穿膜细胞数均增加（P<0.05或0.01）。结论：PM_2.5可增强A549、HepG2和A498细胞的迁移和侵袭能力。更多还原

【Abstract】 OBJECTIVE:To investigate effects of PM_2.5 on the migration and invasion abilities in three types of cancer cells: human lung（A549）, human liver（HepG2） and human renal（A498） cancer cells.METHODS: The A549, HepG2 and A498 cells were exposed to PM_2.5 suspension at 10 and 50 μg/mL for24 h. The transwell assay was performed to detect cell migration and invasion abilities. RESULTS: The results from the migration assay show that the number of transmembrane A549 cells in the negative control,10 and 50 μg/mL PM_2.5 exposure groups were（356.33±30.92）,（476.33±28.50） and（563.33±74.00）,respectively.Data for the HepG2 cells were（86.42±4.75）,（140.00±16.15） and（234.17±9.06）; and those for the A498 cells were（42.33±6.43）,（80.00±6.08） and（174.67±25.01）,respectively. The results from the invasion assay show that the number of transmembrane A549 cells in the three experimental groups were（188.33±9.45）,（313.00±7.94） and（404.00±57.66）,respectively. Data for the HepG2 cells were（48.33±3.26）,（97.75±6.63） and（123.92±8.57）;and those for the A498 cells were（5.67±5.03）,（21.33±5.13） and（40.67±6.81）,respectively. In the migration and invasion experiments,compared with the negative control group,the numbers of penetrating cells of the three types of cells in the PM_2.5 exposure group were significantly increased（P<0.05）. CONCLUSION:PM_2.5 significantly enhanced the abilities of migration and invasion in A549,HepG2 and A498 cells.更多还原