【摘要】 目的观察尿激酶(UK)静脉溶栓对急性脑梗死大鼠的干预效果。方法选取40只SD健康雄性大鼠,选取10只为正常组,其余30只大鼠建立急性脑梗死模型,并随机分为模型组、药物对照组、尿激酶组。正常组、模型组大鼠灌胃生理盐水,药物对照组大鼠静脉注射阿托伐他汀注射液,尿激酶组大鼠给予尿激酶静脉溶栓。比较4组大鼠梗死灶体积、神经功能评分,酶联免疫吸附试验法检测白细胞介素-8(IL-8)、白细胞介素-17(IL-17)、超敏C反应蛋白(hs-CRP)水平,免疫透射比浊法检测超氧化物歧化酶(SOD)、基质金属蛋白酶-3(MMP-3)水平,蛋白免疫印迹(Western Blot)法检测Bcl-2、Bax、Caspase-3相对表达量。结果药物对照组、尿激酶组大鼠梗死灶体积、神经功能评分均低于模型组,且尿激酶组大鼠梗死灶体积、神经功能评分低于药物对照组(P<0.05)。药物对照组、尿激酶组大鼠SOD水平均高于模型组,IL-8、IL-17、hs-CRP、MMP-3水平均低于模型组,且尿激酶组大鼠SOD水平高于药物对照组,IL-8、IL-17、hs-CRP、MMP-3水平低于药物对照组,差异均有统计学意义(P<0.05)。药物对照组、尿激酶组大鼠Bcl-2、Caspase-3表达均低于模型组,Bax表达均高于模型组,且尿激酶组大鼠Bcl-2、Caspase-3表达低于药物对照组,Bax表达高于药物对照组,差异均有统计学意义(P<0.05)。结论使用尿激酶静脉溶栓对急性脑梗死大鼠进行干预,能够有效缩小梗死灶体积,减轻大鼠神经功能受损情况以及脑组织炎性反应,调控SOD、MMP-3水平,抑制海马区细胞凋亡。更多还原

【Abstract】 Objective To explore the effect of intravenous thrombolysis with urokinase(UK) on acute cerebral infarction of rats.Methods Forty SD healthy male rats were selected,10 rats were selected as normal group,the other 30 rats were randomly divided into model group,drug control group and UK intravenous thrombolysis group.The rats in normal group and model group were intervened with normal saline,drug control group rats were intervened with atorvastatin,UK intravenous thrombolysis group rats were intervened with UK intravenous thrombolysis.The infarct volume and neurological score were compared between the four groups.The levels of interleukin-8(IL-8),interleukin-17(IL-17),and hypersensitive C-reactive protein(hs-CRP) were detected by enzyme-linked immunosorbent assay.The levels of superoxide dismutase(SOD) and matrix metalloproteinase-3(MMP-3) were determined by immunoturbidimetry.The Bcl-2,Bax,and Caspase-3 were detected by Western blot.Results The infarct volume and neurological function scores of drug control group and UK intravenous thrombolysis group were lower than those of model group,and the infarct volume and neurological function scores of UK intravenous thrombolysis group were lower than those of the drug control group(P<0.05).The levels of SOD in drug control group and UK intravenous thrombolysis group were higher than those in the model group,the levels of IL-8,IL-17,hs CRP,and MMP-3 in drug control group and UK intravenous thrombolysis group were lower than those in model group,and the level of SOD in UK intravenous thrombolysis group was higher than that in drug control group,the levels of IL-8,IL-17,hs-CRP,and MMP-3 in UK intravenous thrombolysis group were higher than those in drug control group(P<0.05).The expression levels of Bcl-2 and Caspase-3 in drug control group andUK intravenous thrombolysis group were lower than those in model group,Bax expression level was higher than that in model group,and the expression levels of Bcl-2 and Caspase-3 in UK intravenous thrombolysis group were lower than those in drug control group,Baxexpression level was higher than that in drug control group(P<0.05).Conclusion The UK intravenous thrombolysis for acute cerebral infarction of rats can effectively control the volume of infarct,reduce the damage of nerve function and inflammatory response of brain tissue,regulate the level of SOD and MMP-3,and inhibit the apoptosis of hippocampal cells.更多还原