【摘要】 目的应用气相色谱-质谱(GC-MS)分析石菖蒲挥发油成分,并比较其成分含量差异。方法采用水蒸馏法获取石菖蒲根茎部位挥发油后应用GC-MS技术对其进行分离与鉴定,挥发油中各种成分相对含量应用气相色谱面积归一法测定。结果石菖蒲挥发油中各组分通过气相色谱技术分离效果、峰形较好,分析发现挥发油中存在100多种化合物,相对含量高于0.01%化合物有25种,其含量达挥发油总量的93.40%,类型主要为苯丙素衍生类、单萜氧化物类、脂肪醛固酮类以及倍半萜氧化物类4种,其中β-细辛醚、α-细辛醚为含量最高两种化合物,占比分别为54.62%、32.34%;β-细辛醚、α-细辛醚在对应线性范围内呈良好线性关系,两种物质精确性、稳定性、重复性试验相对标准偏差(RSD)低于2.0%;β-细辛醚、α-细辛醚回收率分别为104.2%、101.3%,RSD分别为1.12%、1.83%,应用对应标准曲线测定5个不同批次石菖蒲挥发油中β-细辛醚、α-细辛醚平均含量分别为(535.64±1.4)mg/g、(957.54±0.72)mg/g。结论 GC-MS分析发现安徽产石菖蒲挥发油成分主要有β-细辛醚、α-细辛醚,其中β-细辛醚含量最高。更多还原

【Abstract】 Objective To analyze the constituents of volatile oil in Rhizoma Acori Tatarinowii using gas chromatography-mass spectrometry(GC-MS), and compare the contents of different constituents. Methods The volatile oil was obtained from the Rhizoma Acori Tatarinowii by water distillation and then separated and identified by using GC-MS. The relative contents of various components in volatile oil were determined by gas chromatography area normalizing method. Results The separating effect and peak shape of constituents of volatile oil in Rhizoma Acori Tatarinowii displayed by gas chromatography were relatively better. It was found that there were more than 100 kinds of constituents in the volatile oil, and 25 kinds of constituents with relative content higher than 0.01%, accounting for 93.40% of volatile oil. The 4 main types were phenylpropanoid derivatives, monoterpenoid oxides, fatty aldosterones and sesquiterpene oxides. Among them, the contents of β-asarone and α-asarone were the highest, which were 54.62% and 32.34%. β-asarone and α-asarone had a good linear relationship within the corresponding linear range. The accuracy, stability and repeatability test RSD of the 2 were lower than 2.0%. The recovery rates were 104.2% and 101.3%, the RSD values were 1.12% and 1.83%. The mean contents of β-asarone and α-asarone in volatile oil from 5 different batches of Rhizoma Acori Tatarinowii measured by corresponding standard curves were(535.64±1.4) mg/g and(957.54±0.72) mg/g. Conclusion GC-MS finds that the main constituents of volatile oil in Rhizoma Acori Tatarinowii include β-asarone and α-asarone, and the content of β-asarone is the highest.更多还原