【摘要】 目的:探讨芒柄花黄素对胰腺癌细胞的影响及分子机制。方法:将胰腺癌细胞SW1990分为对照组、芒柄花黄素低、中、高剂量组(10,30,100μmol·L^-1)、si-circ₀020123组、si-NC组、芒柄花黄素(100μmol·L^-1)+pcDNA-circ₀020123组、芒柄花黄素(100μmol·L^-1)+pcDNA组;蛋白质印迹（Western blot）法检测蛋白表达;四甲基偶氮唑盐比色法（MTT）检测细胞增殖抑制率;克隆形成实验检测集落形成数;划痕实验检测细胞划痕愈合率;Transwell检测侵袭细胞数;实时荧光定量PCR（RT-qPCR）检测circ₀020123和miR-145的表达水平;双荧光素酶报告实验检测circ₀020123和miR-145的靶向关系。结果:不同剂量芒柄花黄素处理后,胰腺癌SW1990细胞的增殖抑制率、E-钙黏蛋白（E-cadherin）及miR-145表达水平升高,集落形成数和侵袭细胞数减少,细胞划痕愈合率、N-钙黏蛋白（N-cadherin）及circ₀020123表达水平降低（P<0.05）。干扰circ₀020123表达后,细胞增殖抑制率、E-cadherin及miR-145表达水平升高,集落形成数和侵袭细胞数减少,细胞划痕愈合率、N-cadherin及circ₀020123表达水平降低（P<0.05）。circ₀020123过表达可减弱芒柄花黄素对SW1990增殖迁移侵袭的抑制作用。circ₀020123靶向调控miR-145。结论:芒柄花黄素通过调控circ₀020123/miR-145抑制胰腺癌细胞的增殖与转移。更多还原

【Abstract】 Objective: To explore the effects and its molecular mechanism of formononetin on pancreatic cancer cells. Methods: Pancreatic cancer cells SW1990 were divided into control group, formononetin low, medium and high dose groups(10,30,100 μmol·L^-1), si-circ₀020123 group, si-NC group, formononetin(100 μmol·L^-1)+pcDNA-circ₀020123 group and formononetin(100 μmol·L^-1)+pcDNA group; Western blot was used to detect protein expression; tetramethylazolium salt colorimetric method（MTT） was used to detect cell proliferation inhibition rate; clone formation experiment was used to detect colony formation number; scratch experiment was used to detect cell scratch healing rate; Transwell was employed to detect the number of invaded cells; real-time fluorescent quantitative PCR（RT-qPCR） was used to detect the expression levels of circ₀020123 and miR-145; dual luciferase reporter experiment was used to detect the targeting relationship between circ₀020123 and miR-145.Results: After treatment with different doses of formononetin, the proliferation inhibition rate, E-cadherin and miR-145 expression levels of pancreatic cancer SW1990 cells increased, and the number of colonies formed, number of invasive cells, cell scratch healing rate, N-cadherin and circ₀020123 expression levels decreased（P<0.05）. After interference with the expression of circ₀020123, the proliferation inhibition rate, E-cadherin and miR-145 expression levels increased and the number of colonies formed, number of invasive cells, cell scratch healing rate, N-cadherin and circ₀020123 expression levels decreased（P<0.05）. Overexpression of circ₀020123 could attenuate the inhibitory effects of formononetin on the proliferation, migration and invasion of SW1990. circ₀020123 targeted and regulated miR-145. Conclusion: Formononetin inhibits the proliferation and metastasis of pancreatic cancer cells by regulating circ₀020123/miR-145.更多还原