【摘要】 目的探讨石菖蒲对抑郁大鼠脑内神经递质含量及行为学的影响。方法将30只SD大鼠随机分为空白组、模型组、氟西汀组[1.8 mg/(kg·d)]、石菖蒲低剂量组[5 ml/(kg·d)]、石菖蒲中剂量组[10 ml/(kg·d)]和石菖蒲高剂量组[20 ml/(kg·d)],每组5只。除空白组外,其余5组予以慢性、不可预见的温和刺激(CUMS)建造抑郁大鼠模型。通过强迫游泳试验(FST)、旷场试验(OFT)、高架十字迷宫试验(EPM)比较各组抑郁症状情况。比较各组大鼠脑组织多巴胺(DA)和5-羟色胺(5-HT)水平。结果行为学实验结果显示,模型组游泳不动时间高于空白组,OFT得分、开放臂停留时间(OT)/两臂停留总时间(TT)低于空白组,差异有统计学意义(P <0.05)。模型组脑组织DA、5-HT水平显著低于空白组(P <0.05)。石菖蒲高剂量组、石菖蒲中剂量组、石菖蒲低剂量组、氟西汀组OFT得分、游泳不动时间、OT/TT较模型组改善,差异有统计学意义(P <0.05)。随着石菖蒲剂量的升高,大鼠脑组织DA、5-HT水平均呈上升趋势。石菖蒲高剂量组和氟西汀组脑组织DA、5-HT水平均显著高于模型组(P <0.05),而与空白组比较差异无统计学意义(P> 0.05)。结论石菖蒲通过提高脑组织中的神经递质水平发挥抗抑郁作用。更多还原

【Abstract】 Objective To explore the effects of Acorus calamus on neurotransmitter content in the brain of depression rats and their ethology. Methods Thirty Sprague-Dawley( SD) rats were randomly divided into blank group,model group,fluoxetine group[1. 8 mg/( kg·d) ],Acorus calamus low-dose group[5 ml/( kg·d) ],Acorus calamus middle-dose group[10 ml/( kg·d) ]and Acorus calamus high-dose group[20 ml/( kg·d) ],with 5 rats in each group.Except for the blank group,the other 5 groups were given chronic unpredictable mild stress( CUMS) to establish depression rat models. Forced swimming test( FST),open field test( OFT) and elevated plus-maze test( EPM) were used to compare the depressive symptoms among the 6 groups. The levels of dopamine( DA) and 5-hydroxytryptamine( 5-HT) in brain tissues of rats were compared among the 6 groups. Results The results of behavioral experiments showed that swimming immobility time of the model group was longer than that of the blank group,and that the OFT score and openarm time( OT)/total time( TT) of the model group were lower than those of the blank group,and the differences were statistically significant( P < 0. 05). The levels of DA and 5-HT in brain tissues in the model group were significantly lower than those in the blank group( P < 0. 05). The OFT score,swimming immobility time and OT/TT in the Acorus calamus high-dose group,Acorus calamus middle-dose group,Acorus calamus low-dose group,and the fluoxetine group improved compared with those in the model group,and the differences were statistically significant( P < 0. 05). With the increase of the dose of Acorus calamus,the levels of DA and 5-HT in brain tissues of rats showed an upward trend.The levels of DA and 5-HT in brain tissues in the Acorus calamus high-dose group and the fluoxetine group were significantly higher than those in the model group( P < 0. 05). However,compared with those in the blank group,the levels of DA and 5-HT in the Acorus calamus high-dose group and the fluoxetine group were not significantly different( P >0. 05). Conclusion Acorus calamus exerts an antidepressant effect by increasing the level of neurotransmitters in the brain tissues.更多还原