【摘要】 目的探讨参附注射液功效物质基础及对慢性心力衰竭的作用机制。方法借助中药系统药理学平台(TCMSP)筛选参附注射液中的活性成分和作用靶点,基于Genecards数据库遴选慢性心力衰竭相关的靶点,将中药与疾病靶点进行交集,构建成分-靶点网络;结合String数据库构建成分靶点-疾病靶点蛋白互作全局(PPI)网络,并通过Metascape数据库对重叠靶点进行GO注释及KEGG通路分析。结果本研究共获得17个人参有效成分,5个附子有效成分,119个药物靶点,与慢性心力衰竭相关的78个重叠靶点;GO分子功能富集条目主要与G蛋白偶联受体、神经递质受体、类固醇激素受体、钙条件蛋白相关;GO生物学功能富集条目包括血液循环、神经递质水平调节、细胞凋亡信号通路、跨膜通道离子转运等项;KEGG富集则涵盖流体剪切应力与动脉粥样硬化途径、钙离子信号通路、NF-kappaB信号通路、c AMP信号通路、VEGF信号通路等生物功能。结论本研究结果从分子基因水平初步预测了参附注射液针对慢性心力衰竭的关键通路:增加心肌细1胞肌浆网内Ca2+浓度、增强心肌收缩力、抗心律失常、抑制免疫炎症反应应答与细胞凋亡、抑制血管内皮增生等,从而达到治疗心衰的目的。更多还原

【Abstract】 Objective: To explore the functional material basis of Shenfu Injection and its mechanism on chronic heart failure. Methods: The active ingredients and targets of Shenfu Injection were screened by the TCM System Pharmacology Platform（TCMSP）,the targets related to chronic heart failure were selected based on the Genecards database,and both targets were intersected to construct a component-target Point network;the String database was used to construct a component target-disease target protein interaction（PPI）network,and GO pathway and KEGG pathway for targets above were analyzed through the Metascape database. Results: In this study,a total of 17 active ingredients of ginseng and 5 active ingredients of aconite related to 119 drug targets,with 78 targets overlap with chronic heart failure were obtained;GO molecular function enrichment items were mainly related to G protein coupled receptors,neurotransmitter receptors,steroid hormone receptors,and calcium-conditioned proteins;GO biological function enrichment items included blood circulation,neurotransmitter level regulation,apoptosis signaling pathway,transmembrane channel ion transport,etc.;KEGG enrichment collection covered fluid shear stress and atherosclerosis pathway,calcium ion signaling pathway,NF-kappaB signaling pathway,cAMP signaling pathway,VEGF signaling pathway and other biological functions. Conclusion: This study preliminarily predict the key pathways of Shenfu Injection against chronic heart failure at the molecular gene level,which increase the concentration of Ca²⁺ in the sarcoplasmic reticulum of cardiomyocytes,enhance myocardial contractility,resist arrhythmia,and inhibit immune inflammatory response and cellular apoptosis,inhibit vascular endothelial proliferation,etc.,so as to achieve the purpose of treating heart failure.更多还原