【摘要】 目的探讨小鼠哮喘模型中miRNAs的差异表达情况。方法将24只BALB/c小鼠随机分为正常对照组、卵清蛋白处理组、地塞米松治疗组各8只,使用血细胞分析仪检测每组肺泡灌洗液中嗜酸性粒细胞(EOS)、嗜中性粒细胞(NEU)、巨噬细胞(MAC)和淋巴细胞(LYM)的表达情况,通过ELISA的方法检测每组肺泡灌洗液中白细胞介素-4(IL-4)、IL-6和IL-13的表达情况。miRNAs芯片分析不同组别肺组织中差异性表达miRNAs的情况,统计分析miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的相关性。结果卵清蛋白处理组中EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达显著高于正常对照组,地塞米松治疗组与卵清蛋白处理组相比各种因子表达下调。卵清蛋白处理组多个miRNAs表达发生变化,miR-455在卵清蛋白处理组上调显著。miR-455与EOS、NEU、MAC、LYM、IL-4、IL-6和IL-13的表达呈正相关。结论 miR-455在卵清蛋白诱导的小鼠哮喘模型中显著上调。更多还原

【Abstract】 Objective To investigate the differential expression of miRNAs in asthma model of mice.Methods BALB/c mice were randomly divided into normal control group, asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group), 8 mice in each group. Eosinophil(EOS), neutrophil(NEU), macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4, IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS, NEU, MAC, LYM, IL-4, IL-6 and IL-13 was statistically analyzed.Results The expression of EOS, NEU, MAC, LYM, IL-4, IL-6 and IL-13 in the asthma group was significantly higher than that in the control group, and the expression of various factors was down-regulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed, and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS, NEU, MAC, LYM, IL-4, IL-6 and IL-13.Conclusion miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.更多还原