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一株耐高温黄原胶降解菌的筛选及功能鉴定

Screening and Functional Identification of a Thermostable Xanthan Gum Degradation Strain

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【作者】 胡双喜曹福祥许岗李萌

【Author】 HU Shuangxi;CAO Fuxiang;XU Gang;LI Meng;College of Life Science and Technology, Central South University of Forestry and Technology;

【通讯作者】 李萌;

【机构】 中南林业科技大学生命科学与技术学院

【摘要】 耐高温黄原胶降解菌可为石油工业的破胶技术提供新的菌种资源。本研究从温泉水样中分离出一株耐热黄原胶降解菌,经鉴定,其为地衣芽孢杆菌,最适生长温度为50 ℃,最适生长pH保持在7~8。该菌降解黄原胶的最适条件如下:黄原胶初始浓度为0.2%,pH=7,温度为55 ℃。在此条件下菌株培养36 h时,黄原胶降解酶活性达到最高(64.5 U/L),最大降黏率为58.9%。该菌株在生物破胶领域具有应用潜力。

【Abstract】 High temperature resistant xanthan gum degradation bacteria can provide new bacteria resources for gel breaking technology in the petroleum industry.In this study,a heat-resistant xanthan gum-degrading bacterium was isolated from hot spring water samples,it was identified as Bacillus licheniformis,the optimum growth temperature was 50 ℃,and the optimum growth pH was maintained at 7~8.The optimal conditions for the bacteria to degrade xanthan gum are as follows:the initial concentration of xanthan gum is 0.2%,pH=7,and temperature is 55 ℃.When the strain was cultured for 36 h under these conditions,the activity of xanthan gum degrading enzyme reached the highest (64.5 U/L),and the maximum viscosity reduction rate was 58.9%.The strain has application potential in the field of biological gel breaking.

【基金】 湖南省林业科技创新专项(XLK201984)
  • 【文献出处】 中国资源综合利用 ,China Resources Comprehensive Utilization , 编辑部邮箱 ,2021年04期
  • 【分类号】X172
  • 【被引频次】2
  • 【下载频次】157
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