【摘要】 可可毛色二孢Lasiodiplodia theobromae是引起葡萄溃疡病的主要致病菌之一。病原菌会分泌许多效应子抑制植物的防御反应。本试验对可可毛色二孢特有的效应子LT₃97基因进行克隆,开放阅读框（open reading frame, ORF）全长1 140 bp,含有11个半胱氨酸。生物信息学软件预测显示:效应子LT₃97信号肽为位于N端的1～16个氨基酸。qRT-PCR结果表明:LT₃97受可可毛色二孢诱导下,在侵染24 h的基因相对转录水平最高;在逆境胁迫处理方面:LT₃97在营养、氧化、离子、酸碱和UV胁迫下基因的相对转录水平都上升。烟草瞬时表达表明,效应子LT₃97可抑制水稻细菌性谷枯病菌Burkholderia glumae引发的过敏反应（hypersensitive response, HR）。以上结果为进一步分析效应子对寄主的致病机制奠定了基础。更多还原

【Abstract】 Lasiodiplodia theobromae is one of the important pathogens causing grapevine canker. The effectors produced by the pathogens usually act as inhibitors of plant defense responses and promote successful colonization of the pathogen in plants cells. In this study, the full open reading frame of LT₃97（1 140 bp） was cloned, which included 11 cysteine residues. Bioinformatics analysis predicted that a signal peptide of 1-16 amino acids was located at the N terminus. The results of qRT-PCR showed that LT₃97 was induced by L.theobromae and its relative transcription level was the highest at 24 h after infection. Under stress conditions, the transcription level of LT₃97 gene was increased under nutritional stress, oxidative stress, high-temperature stress and UV treatment. The transient expression of Nicotiana benthamiana indicated that the effector LT₃97 could inhibit the hypersensitive response（HR） induced by Burkholderia glumae. These results provide a reference for further analysis of pathogenesis and the effector interaction with the host.更多还原