【摘要】 目的构建一种吲哚菁绿纳米递药系统用于肿瘤的光热治疗。方法以O-羧甲基壳聚糖和聚乙烯亚胺为载体材料,采用聚电解质复合法,制备了用于肿瘤的光热治疗吲哚菁绿纳米粒(ICG NPs)。并对ICG NPs进行制剂学、光热效能、体外细胞毒性、细胞摄取和荷瘤小鼠抗肿瘤疗效的研究。结果 ICG NPs平均粒径为(142.3±6.53)nm,zeta电位为-(21.7±1.59)mV,包封率与载药量分别为(72.6±3.1)%、(1.5±0.8)%。ICG NPs在808 nm激光照射下可显著升温,呈现浓度依赖性、时间不依赖性的细胞毒性,可明显抑制肿瘤生长。结论 ICG NPs可用于肿瘤的光热治疗,且无明显的毒副作用。更多还原

【Abstract】 Objective To construct an indocyanine green nano-drug delivery system for the photothermal treatment of tumors.Methods O-carboxymethyl chitosan and polyethyleneimine were used as carriers.Indocyanine green nanoparticles (ICG NPs) were prepared by the polyelectrolyte composite method for the photothermal therapy of tumors.The pharmacy characters,photothermal efficacy,in vitro cytotoxicity and cell uptake,and anti-tumor efficacy of ICG NPs in the tumor-bearing mice were determined.Results The average particle size and zeta potential of ICG NPs were (142.3±6.53) nm and-(21.7±1.59) mV,respectively.The encapsulation efficiency and drug loading were (72.6±3.1)% and (1.5±0.8)%,respectively.ICG NPs significantly increased the temperature under 808 nm laser irradiation,exhibiting concentration-dependent and time-independent cytotoxicity,and also inhibited the tumor growth.Conclusion ICG NPs can be used in the photothermal treatment of tumors without obvious side effects.更多还原