【摘要】 为明确玫瑰光合生理变化规律,选择抗寒优良无性系1^#为试验对象,利用紫外分光光度计和Li-6400测定L₃（75%±3%）、L₂（50%±3%）、L₁（25%±3%）、L₀（CK）等4种光照强度下的光合生理特性.结果表明:随着光照强度的减弱,玫瑰叶绿素a、叶绿素b、类胡萝卜素和总叶绿素含量上升,叶绿素a/b下降.L₃、L₁、L₀光照强度下的净光合速率（P_n）日变化为单峰曲线,不存在光合午休,L₂光照强度下的P_n日变化曲线为双峰曲线,存在光合午休;胞间CO₂浓度（C_i）日变化曲线为降低-平缓-上升,呈"V"形,气孔限制值（L_s）日变化曲线为先上升后下降,为倒"V"形;由主成分分析可知,前两个主成分累计贡献率达87.167%;选出P_n、L_s、光合有效辐射（PAR）和蒸腾速率（T_r）等4个光合主导因子进行综合评价,结果可知,L₁光照强度最适合玫瑰进行光合作用.更多还原

【Abstract】 In order to clarify the physiological changes of Rosa rugosa Thunb.,select its excellent cold-resistant clone 1#as the test object,use UV spectrophotometer and Li-6400 to measure photosynthetic physiological characteristics under four light intensities,L₃（ 75%±3%）,L₂（ 50%±3%）,L₁（ 25%±3%）,L₀（ CK）.The results show that the content of chlorophyll a,chlorophyll b,carotenoids and total chlorophyll increases,and chlorophyll a/b decreases with the decrease of light intensity. The daily change of net photosynthetic rates（ P_n） under the light intensity of L₃,L₁ and L₀ is a single-peak curve,and there is no photosynthetic lunch break.The daily change of Pnunder the light intensity of L₂ is a bimodal curve,and there is a photosynthetic lunch break; The daily change curve of intercelluar CO₂ concentration（ C_i） is decreasing-slowly-rising,which is in a“V”shape,and the daily change curve of limitation value（ L_s） is first rising and then falling,which is an inverted “V”shape.According to the principal component analysis,the cumulative contribution rate of the first two principal components reached 87. 167%. Four photosynthetic dominant factors including P_n,L_s,photosynthetic radiation（ PAR） and transpiration rate（ T_r） are seleced to comprehensively evaluate,the results show that L₁ light intensity is the most suitable for photosynthesis of Rosa rugosa Thunb.更多还原