【摘要】 目的基于mRNA高通量测序和生物信息学分析探讨胆囊癌发病机制。方法本研究选取2017年1月至2018年10月在上海东方肝胆外科医院就诊的10例胆囊癌患者。对其胆囊癌组织及癌旁正常组织进行mRNA高通量测序,并进行生物信息学分析。结果肿瘤组织与对应的癌旁正常组织相比,共鉴定出1 704个差异表达基因(DEGs),其中523个DEGs上调,1 181个DEGs下调。其中有674个DEGs显著富集到46个信号通路,包括PI3K-Akt、MAPK、Ras、Wnt等癌症相关信号通路,以及细胞运动相关通路,其中最显著的途径是神经活性配体-受体相互作用信号途径,45个基因显著富集到钙信号通路。结论基于高通量mRNA测序和生物信息学技术分析显示胆囊癌和癌旁正常组织存在DEGs,神经活性的配体-受体相互作用信号通路改变最为显著,钙信号传导途径异常揭示了慢性结石性胆囊炎向胆囊癌转变的重要信号通路。更多还原

【Abstract】 Objective To explore the pathogenesis of gallbladder carcinoma base on high-throughput mRNA sequencing and bioinformatics analysis.Methods 10 patients with gallbladder carcinoma admitted to Shanghai Eastern Hepatobiliary Surgery Hospital from January 2017 to October 2018 were recruited in this study.High-throughput mRNA sequencing and bioinformatics analysis were performed on the gallbladder carcinoma and paracancerous normal tissues.Results 1 704 differentially-expressed genes (DEGs) were identified from tumor tissues and adjacent normal tissues,including 523 DEGs were up-regulated and 1 181 DEGs were down-regulated.Among them,674 DEGs were significantly enriched on 46 signaling pathways,including PI3K-Akt,MAPK,Ras,Wnt and other cancer-related signaling pathways as well as cell movement-related signaling pathways,among which the most significant pathway was neuroactive ligand-receptor interaction signaling pathway,and 45 DEGs were significantly enriched on the calcium signaling pathway.Conclusions High-throughput mRNA sequencing and bioinformatics analysis demonstrate that DEGs exist in gallbladder cancer and adjacent normal tissues.The most significant changes are noted on the neuroactive ligand-receptor interaction signaling pathway.Abnormal calcium signaling pathway is noted as a critical signaling pathway for progression from chronic calculous cholecystitis to gallbladder cancer.更多还原