【摘要】 随着植物中越来越多的MADS-box基因被克隆出来,对经典的ABC模型进一步进行完善,提出花发育的ABCE"四元体模型",其中SEP1、SEP2、SEP3、SEP4为E类基因,SEP1同源基因在花器官识别方面起着重要的作用。本研究以铁皮石斛花器官作为实验材料,用RACE方法快速克隆全长cDNA,生物信息学分析全长cDNA为926 bp,具有完整的开放阅读框(ORF)681 bp,具有227个氨基酸,分离出SEP-A同源基因,另外SEP-B同源基因全长cDNA为954 bp,具有完整的开放阅读框(ORF)774 bp,编码243个氨基酸。在各个花器官中进行RT-PCR,发现SEP-A基因在合蕊柱和唇瓣中表达,而SEP-B基因在唇瓣、花萼和幼叶中表达。更多还原

【Abstract】 As more and more MADS-box genes have been cloned in plants, the classical ABC model has been further improved, and the ABCE "Quaternary Model" of flower development has been proposed.Among them, SEP1,SEP2,SEP3 and SEP4 belong to class E gene, and the SEP1 homologous gene plays an important role in flower organ recognition.In this study, the full-length cDNA of Dendrobium candidum SEP gene was cloned rapidly by RACE method.Bioinformatics analysis showed that the full-length cDNA was 926 bp, with a complete open reading frame(ORF) of 681 bp and SEP protein containing 227 amino acids, and the SEP-A homologous gene was isolated.Additionally, the full-length cDNA of SEP-B homologous gene was 954 bp, with a complete open reading frame(ORF) of 774 bp, encoding 243 amino acids.The results of RT-PCR in various floral organs showed that the SEP-A gene was expressed in the gynostemium and labellum while the SEP-B gene was expressed in the labellum, calyx and young leaves.更多还原