【摘要】 目的观察人粒细胞集落刺激因子(G-CSF)动员前后外周血细胞数量和标记的变化,评估G-CSF动员能力。方法统计2004年3月至2018年12月期间中山市人民医院45例下肢缺血性疾病患者应用G-CSF动员前后外周血中的白细胞、中性粒细胞、单核细胞、红细胞和血小板数量和CD34+标记变化,形成配对资料。结果动员前外周血中的白细胞、中性粒细胞、单核细胞、CD34+细胞绝对计数分别为(8.38±2.21)×10~9/L、(5.26±2.33)×10~9/L、(0.75±0.39)×10~9/L、(9.55±19.35)个/μl,G-CSF动员第3天上升至(40.60±12.90)×10~9/L、(34.88±11.74)×10~9/L、(1.86±0.77)×10~9/L、(33.45±47.42)个/μl,差异均有统计学意义(t=9.230、9.662、4.424、2.732,P=0.000、0.000、0.001、0.021);单核细胞占比动员前为(8.15±1.67)%,动员第3天反降至(4.89±2.13)%,差异有统计学意义(χ2=-5.116,P<0.001)。停止动员后第3天外周血中的白细胞、中性粒细胞、单核细胞、CD34+细胞数量恢复到动员前水平。红细胞、血小板数量动员前后无明显变化,差异无统计学意义(t=1.428、0.538,P=0.184、0.602)。结论 G-CSF动员可以增加外周血中白细胞、中性粒细胞、单核细胞、CD34+细胞的数量,但动员单核细胞能力有限,而红细胞、血小板数量未受明显影响。更多还原

【Abstract】 Objective To assess human granulocyte colony stimulating factor (G-CSF) mobilization capacity through the changes of peripheral blood cell numbers and markers before and after the mobilization of G-CSF.Methods The number and CD34+ markers of leukocytes,neutrophils,monocytes,erythrocytes and platelet cells in peripheral blood cells of 45 patients with lower extremity ischemic diseases from March 2004 to December 2018 in Zhongshan City People’ s Hospital were analyzed before and after G-CSF mobilization.Results The number of leukocytes,neutrophils,monocytes and CD34+ cells in peripheral blood before mobilization were (8.38±2.21)×10~9/L,(5.26±2.33)×10~9/L,(0.75±0.39)×10~9/L,(9.55±19.35) μl,which increased to (40.60±12.90)×10~9/L,(34.88±11.74)×10~9/L,(1.86±0.77)×10~9/L,(33.45±47.42) μl 3 days after mobilization,and the differences were statistically significant (t=9.230,9.662,4.424,2.732,P=0.000,0.000,0.001,0.021).Before mobilization,the proportion of monocytes was (8.15±1.67)%,3 days after mobilization decreased to (4.89±2.13)%,with statistically significant difference (χ2=-5.116,P<0.001).Leukocytes,neutrophils,monocytes,and CD34+ cells in peripheral blood recovered to pre-mobilization levels on the third day after cessation of mobilization.There were no statistically significant differences in the number of erythrocytes and platelet cells before and after mobilization (t=1.428,0.538,P=0.184,0.602).Conclusion G-CSF can mobilize and increase the number of white blood cells,neutrophils,monocytes,CD34+ cells in peripheral blood,but the ability of mobilizing monocytes is limited,while erythrocytes and platelet cells are not affected.更多还原