【摘要】 目的:优化杜仲翅果皮酶解提取液的超滤工艺。方法:采用单因素实验考察超滤膜截留分子量、料液温度、操作压力、操作频率、过膜时间、料液浓度、料液p H对杜仲翅果皮酶解提取液中桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的影响。固定超滤膜截留分子量100 000、料液浓度7 g/L、料液pH 7,以料液温度、操作压力、操作频率、过膜时间为考察因素,以桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的综合得分为评价指标,采用Box-Behnken设计-响应面法对超滤工艺进行优化并验证。结果:杜仲翅果皮酶解提取液的最佳超滤工艺为料液温度35℃、操作压力0.5 MPa、操作频率35 Hz、过膜时间42min。验证试验结果显示,杜仲翅果皮酶解提取液中桃叶珊瑚苷、京尼平苷酸、绿原酸的转移率和固形物去除率的综合得分为78.06%(RSD=1.43%,n=3),与预测值(77.18%)的相对误差为1.14%。结论:优化后的超滤工艺稳定、可靠,可用于杜仲翅果皮酶解提取液的超滤纯化。更多还原

【Abstract】 OBJECTIVE:To optimize the ultrafiltration technology of enzymatic hydrolysate from Eucommia ulmoides peel.METHODS:The single factor test was adopted to investigate the effects of molecular weight of ultrafiltration membrane,liquid temperature,operating pressure,operating frequency,membrane filtration time,liquid concentration and pH on transfer rates of aucubin,geniposide and chlorogenic acid as well as solid removal rate in enzymatic hydrolysate from E. ulmoides peel. Setting the molecular cut off of fixed ultrafiltration membrane of 100 000,liquid concentration of 7 g/L,and p H value of 7,the ultrafiltration technology was optimized by Box-Behnken design response-surface methodology and validated with liquid temperature,operating pressure,operating frequency and membrane passing time as factors,using comprehensive scores calculated from transfer rates of aucubin, geniposide and chlorogenic acid as well as solid removal rate as indexes. RESULTS: The optimal ultrafiltration technology of enzymatic hydrolysate from E. ulmoides peel was as follows as liquid temperature of 35 ℃,operating pressure of 0.5 MPa, operating frequency of 35 Hz and membrane passing time of 42 min. Results of validation tests showed that the comprehensive scores of the transfer rates of aucubin,geniposide and chlorogenic acid as well as solid removal rate in enzymatic hydrolysate from E. ulmoides peel was 78.06%(RSD=1.43%,n=3),and its relative error with the predicted value(77.18%)was 1.14%. CONCLUSIONS:The optimized ultrafiltration technology is stable and reliable,and can be used for the ultrafiltration purification of enzymatic hydrolysate from E. ulmoides peel.更多还原