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一株具有产IAA能力的解淀粉芽孢杆菌的分离鉴定及功能评价

Isolation,Identification and Functional Evaluation of an IAA Producing Bacillus Amyloliquefaciens Strain

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【作者】 陈迪芮凯曾涛田威马瑞谢圣华罗诗彤

【Author】 CHEN Di;RUI Kai;ZENG Tao;TIAN Wei;MA Rui;XIE Shenghua;LUO Shitong;Institute of Plant Protection, Hainan Academy of Agricultural Sciences/Scientific Observation and Experiment Station of Crop Pests in HaiKou, Ministry of Agriculture and Rural Affairs/Key Laboratory of Plant Disease and Pest Control of Hainan Province;

【通讯作者】 芮凯;

【机构】 海南省农业科学院植物保护研究所/农业农村部海口作物有害生物科学观测实验站/海南省植物病虫害防控重点实验室

【摘要】 从槟榔根际土壤和槟榔叶柄中分离细菌,测量它们对槟榔盆栽苗的促生能力。结果表明:筛选到的解淀粉芽孢杆菌菌株DC-7有着明显的促生效果,槟榔盆栽苗的株高、根长、茎粗和鲜重等指标均高于对照,且叶绿素含量、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)等指标均明显优于对照,DC-7菌株的LB发酵液上清中吲哚乙酸(IAA)含量为35.78±1.41ng·mL-1,说明菌株DC-7的促生长作用可能来源于它的产IAA能力。

【Abstract】 Bacteria were isolated from the rhizosphere soil and leaf stalk of areca,and their growth-promoting ability to potted areca seedlings was measured.The results showed that the screened bacillus amyloliquefaciens DC-7 had obvious growth-promoting effect;the plant height,root length,stem diameter,and fresh weight of potted areca seedlings were higher than those of the control;and the chlorophyll content,glutamine synthetase (GS) and glutamate synthase (GOGAT) were significantly better than the control.The IAA content in the supernatant of LB fermentation broth of DC-7 strain was 35.78±1.41 ng·mL-1,indicating that the growth-promoting effect of the strain DC-7 may be due to its IAA producing bility.

【关键词】 槟榔解淀粉芽孢杆菌吲哚乙酸分离鉴定
【Key words】 arecabacillus amyloliquefaciensIAAisolationidentification
【基金】 海南省重大科技计划项目(zdkj201817)
  • 【文献出处】 中国热带农业 ,China Tropical Agriculture , 编辑部邮箱 ,2021年03期
  • 【分类号】S792.91;S714.3
  • 【被引频次】2
  • 【下载频次】694
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